Spontaneous recovery of injured Achilles tendon in inducible nitric oxide synthase gene knockout mice

W. Xia; Y. Wang; R. C. Appleyard; G. A. Smythe; G. A.C. Murrell

doi:10.1007/s00011-005-0006-4

Spontaneous recovery of injured Achilles tendon in inducible nitric oxide synthase gene knockout mice

W. Xia, Y. Wang, R. C. Appleyard, G. A. Smythe, G. A.C. Murrell^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Objective and Design: To determine if inducible nitric oxide synthase (iNOS) gene could affect Achilles tendon healing using iNOS gene knockout mice. Methods: 21 iNOS knockout (iNOS^-/-) mice and 8 of the wild type (iNOS^+/+) mice were utilized in this study. Group 1: iNOS ^+/+ mice (n = 8), group 2: iNOS^-/- mice (n = 11) and group 3: iNOS-/- with a NOS inhibitor, (aminoguanidine, 500 mg/kg/day, via an intraperitoneal mini-osmotic pump for 7 days,n = 10). The right Achilles tendon was transected in all mice and harvested on day 7 for cross-sectional area and biomechanical properties. Serum nitrate concentration of the mice was measured by gas chromatography mass spectrometry (GC/MS). Results: A significant reduction in cross-sectional area of the healing Achilles tendon was observed in group 3 mice compared to group 2 mice (p < 0.01). The serum nitrate concentration in both group 2 and group 3 mice was lower than that in group 1 mice (p < 0.01) iNOS gene deletion and inhibition of NOS did not affect the biomechanical properties of the healing tendons. Conclusions: iNOS gene is not solely responsible for the beneficial effects of nitric oxide (NO) on tendon healing.

Original language	English
Pages (from-to)	40-45
Number of pages	6
Journal	Inflammation Research
Volume	55
Issue number	1
DOIs	https://doi.org/10.1007/s00011-005-0006-4
Publication status	Published - Jan 2006
Externally published	Yes

Keywords

Knockout mice
Nitric oxide synthase
NOS inhibition
Tendon healing

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10.1007/s00011-005-0006-4

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title = "Spontaneous recovery of injured Achilles tendon in inducible nitric oxide synthase gene knockout mice",

abstract = "Objective and Design: To determine if inducible nitric oxide synthase (iNOS) gene could affect Achilles tendon healing using iNOS gene knockout mice. Methods: 21 iNOS knockout (iNOS-/-) mice and 8 of the wild type (iNOS+/+) mice were utilized in this study. Group 1: iNOS +/+ mice (n = 8), group 2: iNOS-/- mice (n = 11) and group 3: iNOS-/- with a NOS inhibitor, (aminoguanidine, 500 mg/kg/day, via an intraperitoneal mini-osmotic pump for 7 days,n = 10). The right Achilles tendon was transected in all mice and harvested on day 7 for cross-sectional area and biomechanical properties. Serum nitrate concentration of the mice was measured by gas chromatography mass spectrometry (GC/MS). Results: A significant reduction in cross-sectional area of the healing Achilles tendon was observed in group 3 mice compared to group 2 mice (p < 0.01). The serum nitrate concentration in both group 2 and group 3 mice was lower than that in group 1 mice (p < 0.01) iNOS gene deletion and inhibition of NOS did not affect the biomechanical properties of the healing tendons. Conclusions: iNOS gene is not solely responsible for the beneficial effects of nitric oxide (NO) on tendon healing.",

keywords = "Knockout mice, Nitric oxide synthase, NOS inhibition, Tendon healing",

author = "W. Xia and Y. Wang and Appleyard, {R. C.} and Smythe, {G. A.} and Murrell, {G. A.C.}",

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AU - Xia, W.

AU - Wang, Y.

AU - Appleyard, R. C.

AU - Smythe, G. A.

AU - Murrell, G. A.C.

PY - 2006/1

Y1 - 2006/1

N2 - Objective and Design: To determine if inducible nitric oxide synthase (iNOS) gene could affect Achilles tendon healing using iNOS gene knockout mice. Methods: 21 iNOS knockout (iNOS-/-) mice and 8 of the wild type (iNOS+/+) mice were utilized in this study. Group 1: iNOS +/+ mice (n = 8), group 2: iNOS-/- mice (n = 11) and group 3: iNOS-/- with a NOS inhibitor, (aminoguanidine, 500 mg/kg/day, via an intraperitoneal mini-osmotic pump for 7 days,n = 10). The right Achilles tendon was transected in all mice and harvested on day 7 for cross-sectional area and biomechanical properties. Serum nitrate concentration of the mice was measured by gas chromatography mass spectrometry (GC/MS). Results: A significant reduction in cross-sectional area of the healing Achilles tendon was observed in group 3 mice compared to group 2 mice (p < 0.01). The serum nitrate concentration in both group 2 and group 3 mice was lower than that in group 1 mice (p < 0.01) iNOS gene deletion and inhibition of NOS did not affect the biomechanical properties of the healing tendons. Conclusions: iNOS gene is not solely responsible for the beneficial effects of nitric oxide (NO) on tendon healing.

AB - Objective and Design: To determine if inducible nitric oxide synthase (iNOS) gene could affect Achilles tendon healing using iNOS gene knockout mice. Methods: 21 iNOS knockout (iNOS-/-) mice and 8 of the wild type (iNOS+/+) mice were utilized in this study. Group 1: iNOS +/+ mice (n = 8), group 2: iNOS-/- mice (n = 11) and group 3: iNOS-/- with a NOS inhibitor, (aminoguanidine, 500 mg/kg/day, via an intraperitoneal mini-osmotic pump for 7 days,n = 10). The right Achilles tendon was transected in all mice and harvested on day 7 for cross-sectional area and biomechanical properties. Serum nitrate concentration of the mice was measured by gas chromatography mass spectrometry (GC/MS). Results: A significant reduction in cross-sectional area of the healing Achilles tendon was observed in group 3 mice compared to group 2 mice (p < 0.01). The serum nitrate concentration in both group 2 and group 3 mice was lower than that in group 1 mice (p < 0.01) iNOS gene deletion and inhibition of NOS did not affect the biomechanical properties of the healing tendons. Conclusions: iNOS gene is not solely responsible for the beneficial effects of nitric oxide (NO) on tendon healing.

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Spontaneous recovery of injured Achilles tendon in inducible nitric oxide synthase gene knockout mice

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