Spontaneous recovery of injured Achilles tendon in inducible nitric oxide synthase gene knockout mice

W. Xia, Y. Wang, R. C. Appleyard, G. A. Smythe, G. A.C. Murrell*

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Citations (Scopus)


Objective and Design: To determine if inducible nitric oxide synthase (iNOS) gene could affect Achilles tendon healing using iNOS gene knockout mice. Methods: 21 iNOS knockout (iNOS-/-) mice and 8 of the wild type (iNOS+/+) mice were utilized in this study. Group 1: iNOS +/+ mice (n = 8), group 2: iNOS-/- mice (n = 11) and group 3: iNOS-/- with a NOS inhibitor, (aminoguanidine, 500 mg/kg/day, via an intraperitoneal mini-osmotic pump for 7 days,n = 10). The right Achilles tendon was transected in all mice and harvested on day 7 for cross-sectional area and biomechanical properties. Serum nitrate concentration of the mice was measured by gas chromatography mass spectrometry (GC/MS). Results: A significant reduction in cross-sectional area of the healing Achilles tendon was observed in group 3 mice compared to group 2 mice (p < 0.01). The serum nitrate concentration in both group 2 and group 3 mice was lower than that in group 1 mice (p < 0.01) iNOS gene deletion and inhibition of NOS did not affect the biomechanical properties of the healing tendons. Conclusions: iNOS gene is not solely responsible for the beneficial effects of nitric oxide (NO) on tendon healing.

Original languageEnglish
Pages (from-to)40-45
Number of pages6
JournalInflammation Research
Issue number1
Publication statusPublished - Jan 2006
Externally publishedYes


  • Knockout mice
  • Nitric oxide synthase
  • NOS inhibition
  • Tendon healing

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