Stable cellulase immobilized on graphene oxide@CMC-g-poly(AMPS-co-AAm) hydrogel for enhanced enzymatic hydrolysis of lignocellulosic biomass

Shohreh Ariaeenejad, Elaheh Motamedi*, Ghasem Hosseini Salekdeh

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)


This study indicated tailoring efficient polymer-enzyme bioconjugates with superb stability and activity for practical utilization of cellulase enzyme in hydrolyzing lignocellulosic biomass. To this goal, a dual crosslinking (DC) strategy was presented to synthesize novel 3D networks of carboxymethyl cellulose grafted copolymers of 2-acrylamido-2methyl propane sulfonate and acrylamide (CMC-g-poly(AMPS-co-AAm)) hydrogels. Graphene oxide (GO) nano-sheets were utilized as nano-filler and physical cross-linker making H-bondings between polymeric chains to prepare GO@CMC-g-poly(AMPS-co-AAm) networks. The GO content effects on the performance of as-synthesized architectures in conjugation to a model enzyme (PersiCel1) were examined. PersiCel1 immobilization on the GO reinforced hydrogels resulted in noticeable retaining near 60 % of its maximum activity at 90 °C, along with the remarkable enhancement of its specific activity and storage stability. Compared with the free PersiCel1, the immobilized enzyme on the GO containing hydrogels showed 154.8 % increase in conversion of alkalin-treated sugar beet pulp, while the PersiCel1/neat-Hydrogel indicated an increment of 66.7 %, under the same conditions.

Original languageEnglish
Article number115661
Pages (from-to)1-12
Number of pages12
JournalCarbohydrate Polymers
Publication statusPublished - 15 Feb 2020



  • Bioconversion
  • Cellulase
  • Dual cross-linked hydrogel
  • Enzyme immobilization
  • Graphene oxide
  • Sugar beet pulp

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