Stage-specific expression of intracisternal a-particle sequences in murine myelomonocytic leukemia cell lines and normal myelomonocytic differentiation

Yoichi Takayama; Margaret Anne O'Mara; Katrina Spilsbury; Rosemary Thwaite; Peter B. Rowe; Geoff Symonds

Stage-specific expression of intracisternal a-particle sequences in murine myelomonocytic leukemia cell lines and normal myelomonocytic differentiation

Yoichi Takayama^*, Margaret Anne O'Mara, Katrina Spilsbury, Rosemary Thwaite, Peter B. Rowe, Geoff Symonds

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The levels of intracisternal A-particle (IAP) mRNA were analyzed in a variety of myelomonocytic leukemia cell lines, peritoneally derived macrophages, and normal hemopoietic progenitors induced to differentiate. both normal and leukemic cells, the highest level of IAP message was found in cells at an intermediate stage of myelomonocytic differentiation, namely, the promyelomonocyte. These results indicate that IAP sequence transcription is regulated differentially during myelomonocytic cell development and that in general, the expression pattern is preserved in leukemic cell lines in vitro. addition, Northern (RNA) analysis detected only type I IAP transcripts as the major IAP message and the expressed IAP subtypes varied in certain cell lines. This is the first comprehensive study of IAP expression in the myelomonocytic lineage and provides a useful system to study the biology of lAPs.

Original language	English
Pages (from-to)	2149-2154
Number of pages	6
Journal	Journal of Virology
Volume	65
Issue number	4
Publication status	Published - 1991

Cite this

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title = "Stage-specific expression of intracisternal a-particle sequences in murine myelomonocytic leukemia cell lines and normal myelomonocytic differentiation",

abstract = "The levels of intracisternal A-particle (IAP) mRNA were analyzed in a variety of myelomonocytic leukemia cell lines, peritoneally derived macrophages, and normal hemopoietic progenitors induced to differentiate. both normal and leukemic cells, the highest level of IAP message was found in cells at an intermediate stage of myelomonocytic differentiation, namely, the promyelomonocyte. These results indicate that IAP sequence transcription is regulated differentially during myelomonocytic cell development and that in general, the expression pattern is preserved in leukemic cell lines in vitro. addition, Northern (RNA) analysis detected only type I IAP transcripts as the major IAP message and the expressed IAP subtypes varied in certain cell lines. This is the first comprehensive study of IAP expression in the myelomonocytic lineage and provides a useful system to study the biology of lAPs.",

author = "Yoichi Takayama and O'Mara, {Margaret Anne} and Katrina Spilsbury and Rosemary Thwaite and Rowe, {Peter B.} and Geoff Symonds",

year = "1991",

language = "English",

volume = "65",

pages = "2149--2154",

journal = "Journal of Virology",

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publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Stage-specific expression of intracisternal a-particle sequences in murine myelomonocytic leukemia cell lines and normal myelomonocytic differentiation

AU - Takayama, Yoichi

AU - O'Mara, Margaret Anne

AU - Spilsbury, Katrina

AU - Thwaite, Rosemary

AU - Rowe, Peter B.

AU - Symonds, Geoff

PY - 1991

Y1 - 1991

N2 - The levels of intracisternal A-particle (IAP) mRNA were analyzed in a variety of myelomonocytic leukemia cell lines, peritoneally derived macrophages, and normal hemopoietic progenitors induced to differentiate. both normal and leukemic cells, the highest level of IAP message was found in cells at an intermediate stage of myelomonocytic differentiation, namely, the promyelomonocyte. These results indicate that IAP sequence transcription is regulated differentially during myelomonocytic cell development and that in general, the expression pattern is preserved in leukemic cell lines in vitro. addition, Northern (RNA) analysis detected only type I IAP transcripts as the major IAP message and the expressed IAP subtypes varied in certain cell lines. This is the first comprehensive study of IAP expression in the myelomonocytic lineage and provides a useful system to study the biology of lAPs.

AB - The levels of intracisternal A-particle (IAP) mRNA were analyzed in a variety of myelomonocytic leukemia cell lines, peritoneally derived macrophages, and normal hemopoietic progenitors induced to differentiate. both normal and leukemic cells, the highest level of IAP message was found in cells at an intermediate stage of myelomonocytic differentiation, namely, the promyelomonocyte. These results indicate that IAP sequence transcription is regulated differentially during myelomonocytic cell development and that in general, the expression pattern is preserved in leukemic cell lines in vitro. addition, Northern (RNA) analysis detected only type I IAP transcripts as the major IAP message and the expressed IAP subtypes varied in certain cell lines. This is the first comprehensive study of IAP expression in the myelomonocytic lineage and provides a useful system to study the biology of lAPs.

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M3 - Article

C2 - 1848323

AN - SCOPUS:0026149343

SN - 0022-538X

VL - 65

SP - 2149

EP - 2154

JO - Journal of Virology

JF - Journal of Virology

IS - 4

ER -

Stage-specific expression of intracisternal a-particle sequences in murine myelomonocytic leukemia cell lines and normal myelomonocytic differentiation

Abstract

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