Standardized peripheral blood mononuclear cell culture assay for zidovudine susceptibility testing of clinical human immunodeficiency virus type 1 isolates

Effect of reducing the numbers of replicates and concentrations

I. C. Marschner*, D. L. Mayers, A. Erice, L. Smeaton, V. A. Johnson, D. D. Richman, P. Reichelderfer, A. J. Japour

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Zidovudine susceptibility was assessed for 525 clinical human immunodeficiency virus type 1 isolates, before and after reducing the number of replicates and zidovudine concentrations in the standardized consensus peripheral blood mononuclear cell culture assay. We conclude that omitting the 0.001 μM concentration and using duplicate rather than triplicate wells are valid and cost-effective modifications of this expensive assay.

Original languageEnglish
Pages (from-to)756-758
Number of pages3
JournalJournal of Clinical Microbiology
Volume35
Issue number3
Publication statusPublished - 1997
Externally publishedYes

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