Strain-specific and tissue-specific expression of mouse mast cell secretory granule proteases

Richard L. Stevens*, Daniel S. Friend, H. Patrick Mcneil, Vera Schiller, Namit Ghildyal, K. Frank Austen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

112 Citations (Scopus)

Abstract

As assessed by RNA blot analyses with gene-specific probes, we report that the perivascular connective tissue mast cells (CTMCs) in the ear and skin of BALB/cJ mice contain abundant levels of the mouse mast cell protease (mMCP) 7 transcript, in addition to those protease transcripts present in their serosal mast cells (SMCs). High levels of the mMCP-7 transcript also were detected in the ears of WBB6F1/J-+/+, WCB6F1/J-+/+, WB/ReJ-+/+, and WC/ReJ- +/+ mice. However, the ears of these four strains and the SMCs from the WCB6F1/J-+/+ strain but not the BALB/cJ strain also contained high steady- state levels of the mMCP-2 transcript. The mMCP-2, mMCP-4, mMCP-5, mMCP-6, and mMCP-7 transcripts were not detected in the ears of mast-cell-deficient WBB6F1/J-W/W(v) and WCB6F1/J-Sl/Sl(d) mice, indicating that mast cells were the source of these protease transcripts in the +/+ animals. When immunohistochemical analyses of serial sections of ear and skin from WBB6F1/J-+/+ mice were performed with anti-mMCP-2 IgG and anti-mMCP-5 IgG, the perivascular CTMCs in these tissues were found to express both mMCP-2 and mMCP-5 in their granules. The prominent expression of mMCP-7 in constitutive perivascular CTMCs indicates that this mast cell has an extended protease phenotype relative to the SMCs of the same strains. Further, the perivascular CTMCs and SMCs of the +/+ strains differ from those in BALB/cJ mice in their prominent expression of mMCP-2.

Original languageEnglish
Pages (from-to)128-132
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number1
DOIs
Publication statusPublished - 4 Jan 1994
Externally publishedYes

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