Strategies for fabricating a biorecognition interface for a label free electrochemical immunosensor

Sook Mei Khor; Guozhen Liu; Jason B. Harper; Sridhar G. Iyengar; J. Justin Gooding

doi:10.1109/ICONN.2010.6045168

Strategies for fabricating a biorecognition interface for a label free electrochemical immunosensor

Sook Mei Khor^*, Guozhen Liu, Jason B. Harper, Sridhar G. Iyengar, J. Justin Gooding

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

2 Citations (Scopus)

Abstract

An electrochemical interface for a label-free electrochemical immuno-biosensor was prepared by modifying electrodes with a mixed layer comprising a molecular wire (MW) and oligo(ethylene glycol) (OEG) molecules derived from in situ-generated aryl diazonium salts. The in situ-generated aryl diazonium salts were prepared in two different ways using either organic solvents or aqueous solutions. For organic phase surface modification, the corresponding amines of MW and OEG were dissolved in acetonitrile while for aqueous phase surface modification the corresponding amines of MW and OEG were dissolved in 0.5 M HCl acidic solution. Reduction of the aryl diazonium cations generates the corresponding aryl radicals which react with the carbon substrate, resulting in a covalent bond. Our results show that the interface generated in acidic aqueous conditions not only produced denser layers on the electrode surfaces, as determined by the passivation of the electrode towards the redox active species such as Fe(CN)₆ ^3/4 and Ru(NH ₃)₆ ^2/3, but also has good responses for the detection of small molecules such as biotin.

Original language	English
Title of host publication	ICONN 2010 - Proceedings of the 2010 International Conference on Nanoscience and Nanotechnology
Place of Publication	Piscataway, NJ
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
Pages	230-233
Number of pages	4
ISBN (Print)	9781424452620
DOIs	https://doi.org/10.1109/ICONN.2010.6045168
Publication status	Published - 2010
Externally published	Yes
Event	2010 3rd International Conference on Nanoscience and Nanotechnology, ICONN - 2010 - Sydney, Australia Duration: 22 Feb 2010 → 26 Feb 2010

Other

Other	2010 3rd International Conference on Nanoscience and Nanotechnology, ICONN - 2010
Country/Territory	Australia
City	Sydney
Period	22/02/10 → 26/02/10

Keywords

Aryl diazonium salts
Label-free electrochemical immuno-biosensor
Molecular wire
Oligo(ethylene glycol)

Access to Document

10.1109/ICONN.2010.6045168

Cite this

Khor, S. M., Liu, G., Harper, J. B., Iyengar, S. G., & Gooding, J. J. (2010). Strategies for fabricating a biorecognition interface for a label free electrochemical immunosensor. In ICONN 2010 - Proceedings of the 2010 International Conference on Nanoscience and Nanotechnology (pp. 230-233). Article 6045168 Institute of Electrical and Electronics Engineers (IEEE). https://doi.org/10.1109/ICONN.2010.6045168

@inproceedings{cfa8f0828191483386f138d9951e92d9,

title = "Strategies for fabricating a biorecognition interface for a label free electrochemical immunosensor",

abstract = "An electrochemical interface for a label-free electrochemical immuno-biosensor was prepared by modifying electrodes with a mixed layer comprising a molecular wire (MW) and oligo(ethylene glycol) (OEG) molecules derived from in situ-generated aryl diazonium salts. The in situ-generated aryl diazonium salts were prepared in two different ways using either organic solvents or aqueous solutions. For organic phase surface modification, the corresponding amines of MW and OEG were dissolved in acetonitrile while for aqueous phase surface modification the corresponding amines of MW and OEG were dissolved in 0.5 M HCl acidic solution. Reduction of the aryl diazonium cations generates the corresponding aryl radicals which react with the carbon substrate, resulting in a covalent bond. Our results show that the interface generated in acidic aqueous conditions not only produced denser layers on the electrode surfaces, as determined by the passivation of the electrode towards the redox active species such as Fe(CN)6 3/4 and Ru(NH 3)6 2/3, but also has good responses for the detection of small molecules such as biotin.",

keywords = "Aryl diazonium salts, Label-free electrochemical immuno-biosensor, Molecular wire, Oligo(ethylene glycol)",

author = "Khor, {Sook Mei} and Guozhen Liu and Harper, {Jason B.} and Iyengar, {Sridhar G.} and Gooding, {J. Justin}",

year = "2010",

doi = "10.1109/ICONN.2010.6045168",

language = "English",

isbn = "9781424452620",

pages = "230--233",

booktitle = "ICONN 2010 - Proceedings of the 2010 International Conference on Nanoscience and Nanotechnology",

publisher = "Institute of Electrical and Electronics Engineers (IEEE)",

address = "United States",

note = "2010 3rd International Conference on Nanoscience and Nanotechnology, ICONN - 2010 ; Conference date: 22-02-2010 Through 26-02-2010",

}

Khor, SM, Liu, G, Harper, JB, Iyengar, SG & Gooding, JJ 2010, Strategies for fabricating a biorecognition interface for a label free electrochemical immunosensor. in ICONN 2010 - Proceedings of the 2010 International Conference on Nanoscience and Nanotechnology., 6045168, Institute of Electrical and Electronics Engineers (IEEE), Piscataway, NJ, pp. 230-233, 2010 3rd International Conference on Nanoscience and Nanotechnology, ICONN - 2010, Sydney, New South Wales, Australia, 22/02/10. https://doi.org/10.1109/ICONN.2010.6045168

Strategies for fabricating a biorecognition interface for a label free electrochemical immunosensor. / Khor, Sook Mei; Liu, Guozhen; Harper, Jason B. et al.
ICONN 2010 - Proceedings of the 2010 International Conference on Nanoscience and Nanotechnology. Piscataway, NJ: Institute of Electrical and Electronics Engineers (IEEE), 2010. p. 230-233 6045168.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

TY - GEN

T1 - Strategies for fabricating a biorecognition interface for a label free electrochemical immunosensor

AU - Khor, Sook Mei

AU - Liu, Guozhen

AU - Harper, Jason B.

AU - Iyengar, Sridhar G.

AU - Gooding, J. Justin

PY - 2010

Y1 - 2010

N2 - An electrochemical interface for a label-free electrochemical immuno-biosensor was prepared by modifying electrodes with a mixed layer comprising a molecular wire (MW) and oligo(ethylene glycol) (OEG) molecules derived from in situ-generated aryl diazonium salts. The in situ-generated aryl diazonium salts were prepared in two different ways using either organic solvents or aqueous solutions. For organic phase surface modification, the corresponding amines of MW and OEG were dissolved in acetonitrile while for aqueous phase surface modification the corresponding amines of MW and OEG were dissolved in 0.5 M HCl acidic solution. Reduction of the aryl diazonium cations generates the corresponding aryl radicals which react with the carbon substrate, resulting in a covalent bond. Our results show that the interface generated in acidic aqueous conditions not only produced denser layers on the electrode surfaces, as determined by the passivation of the electrode towards the redox active species such as Fe(CN)6 3/4 and Ru(NH 3)6 2/3, but also has good responses for the detection of small molecules such as biotin.

AB - An electrochemical interface for a label-free electrochemical immuno-biosensor was prepared by modifying electrodes with a mixed layer comprising a molecular wire (MW) and oligo(ethylene glycol) (OEG) molecules derived from in situ-generated aryl diazonium salts. The in situ-generated aryl diazonium salts were prepared in two different ways using either organic solvents or aqueous solutions. For organic phase surface modification, the corresponding amines of MW and OEG were dissolved in acetonitrile while for aqueous phase surface modification the corresponding amines of MW and OEG were dissolved in 0.5 M HCl acidic solution. Reduction of the aryl diazonium cations generates the corresponding aryl radicals which react with the carbon substrate, resulting in a covalent bond. Our results show that the interface generated in acidic aqueous conditions not only produced denser layers on the electrode surfaces, as determined by the passivation of the electrode towards the redox active species such as Fe(CN)6 3/4 and Ru(NH 3)6 2/3, but also has good responses for the detection of small molecules such as biotin.

KW - Aryl diazonium salts

KW - Label-free electrochemical immuno-biosensor

KW - Molecular wire

KW - Oligo(ethylene glycol)

UR - http://www.scopus.com/inward/record.url?scp=80555156721&partnerID=8YFLogxK

U2 - 10.1109/ICONN.2010.6045168

DO - 10.1109/ICONN.2010.6045168

M3 - Conference proceeding contribution

AN - SCOPUS:80555156721

SN - 9781424452620

SP - 230

EP - 233

BT - ICONN 2010 - Proceedings of the 2010 International Conference on Nanoscience and Nanotechnology

PB - Institute of Electrical and Electronics Engineers (IEEE)

CY - Piscataway, NJ

T2 - 2010 3rd International Conference on Nanoscience and Nanotechnology, ICONN - 2010

Y2 - 22 February 2010 through 26 February 2010

ER -

Khor SM, Liu G, Harper JB, Iyengar SG, Gooding JJ. Strategies for fabricating a biorecognition interface for a label free electrochemical immunosensor. In ICONN 2010 - Proceedings of the 2010 International Conference on Nanoscience and Nanotechnology. Piscataway, NJ: Institute of Electrical and Electronics Engineers (IEEE). 2010. p. 230-233. 6045168 doi: 10.1109/ICONN.2010.6045168

Strategies for fabricating a biorecognition interface for a label free electrochemical immunosensor

Abstract

Other

Keywords

Access to Document

Other files and links

Fingerprint

Cite this