Stress-induced mutation rates show a sigmoidal and saturable increase due to the RpoS sigma factor in Escherichia coli

Ram Maharjan; Thomas Ferenci

doi:10.1534/genetics.114.170258

Stress-induced mutation rates show a sigmoidal and saturable increase due to the RpoS sigma factor in Escherichia coli

Ram Maharjan, Thomas Ferenci

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Stress-induced mutagenesis was investigated in the absence of selection for growth fitness by using synthetic biology to control perceived environmental stress in Escherichia coli. We find that controlled intracellular RpoS dosage is central to a sigmoidal, saturable three- to fourfold increase in mutation rates and associated changes in DNA repair proteins.

Original language	English
Pages (from-to)	1231-1235
Number of pages	5
Journal	Genetics
Volume	198
Issue number	3
DOIs	https://doi.org/10.1534/genetics.114.170258
Publication status	Published - Nov 2014
Externally published	Yes

Keywords

Bacterial Proteins/metabolism
Escherichia coli/genetics
Gene Dosage
Intracellular Space/metabolism
Mutation Rate
Sigma Factor/metabolism
Stress, Physiological/genetics

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10.1534/genetics.114.170258

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abstract = "Stress-induced mutagenesis was investigated in the absence of selection for growth fitness by using synthetic biology to control perceived environmental stress in Escherichia coli. We find that controlled intracellular RpoS dosage is central to a sigmoidal, saturable three- to fourfold increase in mutation rates and associated changes in DNA repair proteins. ",

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KW - Bacterial Proteins/metabolism

KW - Escherichia coli/genetics

KW - Gene Dosage

KW - Intracellular Space/metabolism

KW - Mutation Rate

KW - Sigma Factor/metabolism

KW - Stress, Physiological/genetics

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Stress-induced mutation rates show a sigmoidal and saturable increase due to the RpoS sigma factor in Escherichia coli

Abstract

Keywords

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