Structural and functional implications of tau hyperphosphorylation

information from phosphorylation-mimicking mutated tau proteins

Jochen Eidenmuller, Thomas Fath, Andrea Hellwig, Jennifer Reed, Estelle Sontag, Roland Brandt

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Abnormal tau-immunoreactive filaments are a hallmark of tauopathies, including Alzheimer's disease (AD). A higher phosphorylation ('hyperphosphorylation') state of tau protein may represent a critical event. To determine the potential role of tau hyperphosphorylation in these disorders, mutated tau proteins were produced where serine/threonine residues known to be highly phosphorylated in tan filaments isolated from AD patients were substituted for glutamate to simulate a paired helical filament (PHF)-like tau hyperphosphorylation. We demonstrate that, like hyperphosphorylation, glutamate substitutions induce compact structure elements and SDS-resistant conformational domains in tau protein. Hyperphosphorylation-mimicking glutamate-mutated tau proteins display a complete functional loss in its ability to promote microtubule nucleation which can partially be overcome by addition of the osmolyte trimethylamine N-oxide (TMAO), which is similar to phosphorylated tau. In addition, glutamate-mutated tan proteins fail to interact with the dominant brain protein phosphatase 2A isoform ABαC, and exhibit a reduced ability to assemble into filaments. Interestingly, wild-type tau and phosphorylation-mimicking tan similarly bind to microtubules when added alone, but the mutated tan is almost completely displaced from the microtubule surface by equimolar concentrations of wild-type tau. The data indicate that glutamate-mutated tau proteins provide a useful model for analyzing the functional consequences of tau hyperphosphorylation. They suggest that several mechanisms contribute to the abnormal tau accumulation observed during tauopathies, in particular a selective displacement of hyperphosphorylated tau from microtubules, a functional loss in promoting microtubule nucleation, and a failure to interact with phosphatases.

Original languageEnglish
Pages (from-to)13166-13175
Number of pages10
JournalBiochemistry
Volume39
Issue number43
DOIs
Publication statusPublished - 8 Nov 2000
Externally publishedYes

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