Subcellular shotgun proteomics in plants: Looking beyond the usual suspects

Paul A. Haynes*, Thomas H. Roberts

*Corresponding author for this work

    Research output: Contribution to journalReview articlepeer-review

    52 Citations (Scopus)


    In this review we examine the current state of analytical methods used for shotgun proteomics experiments in plants. The rapid advances in this field in recent years are discussed, and contrasted with experiments performed using current widely used procedures. We also examine the use of subcellular fractionation approaches as they apply to plant proteomics, and discuss how appropriate sample preparation can produce a great increase in proteome coverage in subsequent analysis. We conclude that the conjunction of these two techniques represents a significant advance in plant proteomics, and the future of plant biology research will continue to be enriched by the ongoing development of proteomic analytical technology.

    Original languageEnglish
    Pages (from-to)2963-2975
    Number of pages13
    Issue number16
    Publication statusPublished - Aug 2007


    Dive into the research topics of 'Subcellular shotgun proteomics in plants: Looking beyond the usual suspects'. Together they form a unique fingerprint.

    Cite this