Subcellular shotgun proteomics in plants

Looking beyond the usual suspects

Paul A. Haynes*, Thomas H. Roberts

*Corresponding author for this work

Research output: Contribution to journalReview article

52 Citations (Scopus)

Abstract

In this review we examine the current state of analytical methods used for shotgun proteomics experiments in plants. The rapid advances in this field in recent years are discussed, and contrasted with experiments performed using current widely used procedures. We also examine the use of subcellular fractionation approaches as they apply to plant proteomics, and discuss how appropriate sample preparation can produce a great increase in proteome coverage in subsequent analysis. We conclude that the conjunction of these two techniques represents a significant advance in plant proteomics, and the future of plant biology research will continue to be enriched by the ongoing development of proteomic analytical technology.

Original languageEnglish
Pages (from-to)2963-2975
Number of pages13
JournalProteomics
Volume7
Issue number16
DOIs
Publication statusPublished - Aug 2007

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