Superoxide detection by chemiluminescence, fluorescence, and spectrophotometry methods

Anindya P. Susanto, Mohammad Firdhaosh, Dong-An Wang, Kyaw Thu Moe

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

1 Citation (Scopus)

Abstract

Pathology of vessel wall endothelium has been attributed to the presence of reactive oxygen species (ROS) and its effects during the onset of oxidative stress. The root of all ROS produced is superoxide (O2*-). Hence, its detection is imperative in filtering out appropriate antioxidants and obtaining deeper knowledge on its role. The present study used lucigenin-enhanced chemiluminescence, hydroethidine fluorescence, and cytochrome c spectrophotometric assays to measure O2*-. The aim was to compare and contrast among the methods using human embryonic kidney cells (HEK). Superoxide production was induced by NADH via NAD(P)H oxidase system. The study showed that lucigenin enhanced chemiluminescence assay was the most sensitive method whilst hydroethidine and cytochrome c assays were proficient as well.
Original languageEnglish
Title of host publicationICBPE 2006
Subtitle of host publicationProceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering
Place of PublicationSingapore
PublisherResearch Publishing Services
Pages537-540
Number of pages4
ISBN (Electronic)8190426249
ISBN (Print)9789810579
Publication statusPublished - 2006
Externally publishedYes
Event2006 International Conference on Biomedical and Pharmaceutical Engineering: ICBPE 2006 - , Singapore
Duration: 11 Dec 200614 Dec 2006

Conference

Conference2006 International Conference on Biomedical and Pharmaceutical Engineering
CountrySingapore
Period11/12/0614/12/06

Keywords

  • free radicals
  • superoxide
  • lucigenin
  • hydroethidine
  • cytochrome c

Fingerprint

Dive into the research topics of 'Superoxide detection by chemiluminescence, fluorescence, and spectrophotometry methods'. Together they form a unique fingerprint.

Cite this