Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy

Menuk B. Jayawardena; Lachlan H. Yee; Ian J. Rainbow; Peter Bergquist; Christopher Such; Peter D. Steinberg; Staffan J. Kjelleberg

doi:10.1016/j.colsurfb.2010.08.042

Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy

Menuk B. Jayawardena, Lachlan H. Yee^*, Ian J. Rainbow, Peter Bergquist, Christopher Such, Peter D. Steinberg, Staffan J. Kjelleberg

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple (TM), was demonstrated as a technique for the visualization of Thermomyces (Humicola) lanuginosa lipase (LIP(HLL)) and Candida antarctica lipase A (LIP(CA)) within a transparent latex coating. Addition of Teric Surfactants (C(16) non-ionic Teric 475, 1.8% (w/w) or C(10) non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIP(HLL) and LIP(CA) to the surface of a latex coating. An alpha-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2 mu m of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating. (C) 2010 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	291-296
Number of pages	6
Journal	Colloids and Surfaces B: Biointerfaces
Volume	82
Issue number	2
DOIs	https://doi.org/10.1016/j.colsurfb.2010.08.042
Publication status	Published - 1 Feb 2011

Keywords

Confocal laser scanning microscopy
Lipase
Catalytic coating
Surface enzyme activity
Surfactant
Latex
COATINGS
EPICOCCONONE
IMMOBILIZATION
MECHANISM
SILICA

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10.1016/j.colsurfb.2010.08.042

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title = "Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy",

abstract = "Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple (TM), was demonstrated as a technique for the visualization of Thermomyces (Humicola) lanuginosa lipase (LIP(HLL)) and Candida antarctica lipase A (LIP(CA)) within a transparent latex coating. Addition of Teric Surfactants (C(16) non-ionic Teric 475, 1.8% (w/w) or C(10) non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIP(HLL) and LIP(CA) to the surface of a latex coating. An alpha-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2 mu m of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating. (C) 2010 Elsevier B.V. All rights reserved.",

keywords = "Confocal laser scanning microscopy, Lipase, Catalytic coating, Surface enzyme activity, Surfactant, Latex, COATINGS, EPICOCCONONE, IMMOBILIZATION, MECHANISM, SILICA",

author = "Jayawardena, {Menuk B.} and Yee, {Lachlan H.} and Rainbow, {Ian J.} and Peter Bergquist and Christopher Such and Steinberg, {Peter D.} and Kjelleberg, {Staffan J.}",

year = "2011",

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language = "English",

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pages = "291--296",

journal = "Colloids and Surfaces B: Biointerfaces",

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T1 - Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy

AU - Jayawardena, Menuk B.

AU - Yee, Lachlan H.

AU - Rainbow, Ian J.

AU - Bergquist, Peter

AU - Such, Christopher

AU - Steinberg, Peter D.

AU - Kjelleberg, Staffan J.

PY - 2011/2/1

Y1 - 2011/2/1

N2 - Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple (TM), was demonstrated as a technique for the visualization of Thermomyces (Humicola) lanuginosa lipase (LIP(HLL)) and Candida antarctica lipase A (LIP(CA)) within a transparent latex coating. Addition of Teric Surfactants (C(16) non-ionic Teric 475, 1.8% (w/w) or C(10) non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIP(HLL) and LIP(CA) to the surface of a latex coating. An alpha-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2 mu m of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating. (C) 2010 Elsevier B.V. All rights reserved.

AB - Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple (TM), was demonstrated as a technique for the visualization of Thermomyces (Humicola) lanuginosa lipase (LIP(HLL)) and Candida antarctica lipase A (LIP(CA)) within a transparent latex coating. Addition of Teric Surfactants (C(16) non-ionic Teric 475, 1.8% (w/w) or C(10) non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIP(HLL) and LIP(CA) to the surface of a latex coating. An alpha-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2 mu m of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating. (C) 2010 Elsevier B.V. All rights reserved.

KW - Confocal laser scanning microscopy

KW - Lipase

KW - Catalytic coating

KW - Surface enzyme activity

KW - Surfactant

KW - Latex

KW - COATINGS

KW - EPICOCCONONE

KW - IMMOBILIZATION

KW - MECHANISM

KW - SILICA

U2 - 10.1016/j.colsurfb.2010.08.042

DO - 10.1016/j.colsurfb.2010.08.042

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JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

IS - 2

ER -

Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy

Abstract

Keywords

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