Synthesis and application of an aldazine-based fluorescence chemosensor for the sequential detection of cu2+ and biological thiols in aqueous solution and living cells

Hongmin Jia, Ming Yang, Qingtao Meng*, Guangjie He, Yue Wang, Zhizhi Hu, Run Zhang, Zhiqiang Zhang

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Citations (Scopus)
14 Downloads (Pure)

Abstract

A fluorescence chemosensor, 2-hydroxy-1-naphthaldehyde azine (HNA) was designed and synthesized for sequential detection of Cu2+ and biothiols. It was found that HNA can specifically bind to Cu2+ with 1:1 stoichiometry, accompanied with a dramatic fluorescence quenching and a remarkable bathochromic-shift of the absorbance peak in HEPES buffer. The generated HNA-Cu2+ensemble displayed a “turn-on” fluorescent response specific for biothiols (Hcy, Cys and GSH) based on the displacement approach, giving a remarkable recovery of fluorescence and UV-Vis spectra. The detection limits of HNA-Cu2+ to Hcy, Cys and GSH were estimated to be 1.5 μM, 1.0 μM and 0.8 μM, respectively, suggesting that HNA-Cu2+ is sensitive enough for the determination of thiols in biological systems. The biocompatibility of HNA towards A549 human lung carcinoma cell, was evaluated by an MTT assay. The capability of HNA-Cu2+ to detect biothiols in live A549 cells was then demonstrated by a microscopy fluorescence imaging assay.

Original languageEnglish
Article number79
Pages (from-to)1-14
Number of pages14
JournalSensors (Switzerland)
Volume16
Issue number1
DOIs
Publication statusPublished - 11 Jan 2016

Bibliographical note

Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • Aldazine
  • Biothiols
  • Chemosensing ensemble
  • Detection
  • Fluorescence imaging

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