A method for the synthesis of deuterium‐labelled tryptophan and its enzymatic conversion to indole‐3‐pyruvic acid ((2H5)IPYA) using amino acid oxidase is described. (2H5)IPYA can be used as an internal standard for the quantitation of IPYA by mass spectrometry in plant extracts and for estimating the breakdown of IPYA to indole‐3‐acetic acid (IAA) during sample extraction and purification. When used in conjunction with (2H2) IAA, (2H5) IPYA should allow more accurate quantitation of IAA in plant extracts.
|Number of pages||6|
|Journal||Journal of Labelled Compounds and Radiopharmaceuticals|
|Publication status||Published - 1983|
- deuterium‐labelled indole‐3‐pyruvic acid
- indole‐3‐acetic acid
- mass spectrometry