Synthesis of (2H5) indole‐3‐pyruvic acid

Jane Badenoch‐Jones; Roger E. Summons; David S. Letham

doi:10.1002/jlcr.2580201203

Synthesis of (²H₅) indole‐3‐pyruvic acid

Jane Badenoch‐Jones^*, Roger E. Summons, David S. Letham

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A method for the synthesis of deuterium‐labelled tryptophan and its enzymatic conversion to indole‐3‐pyruvic acid ((²H₅)IPYA) using amino acid oxidase is described. (²H₅)IPYA can be used as an internal standard for the quantitation of IPYA by mass spectrometry in plant extracts and for estimating the breakdown of IPYA to indole‐3‐acetic acid (IAA) during sample extraction and purification. When used in conjunction with (²H₂) IAA, (²H₅) IPYA should allow more accurate quantitation of IAA in plant extracts.

Original language	English
Pages (from-to)	1325-1330
Number of pages	6
Journal	Journal of Labelled Compounds and Radiopharmaceuticals
Volume	20
Issue number	12
DOIs	https://doi.org/10.1002/jlcr.2580201203
Publication status	Published - 1983

Keywords

deuterium‐labelled indole‐3‐pyruvic acid
indole‐3‐acetic acid
mass spectrometry

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10.1002/jlcr.2580201203

Link to publication in Scopus

Cite this

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title = "Synthesis of (2H5) indole‐3‐pyruvic acid",

abstract = "A method for the synthesis of deuterium‐labelled tryptophan and its enzymatic conversion to indole‐3‐pyruvic acid ((2H5)IPYA) using amino acid oxidase is described. (2H5)IPYA can be used as an internal standard for the quantitation of IPYA by mass spectrometry in plant extracts and for estimating the breakdown of IPYA to indole‐3‐acetic acid (IAA) during sample extraction and purification. When used in conjunction with (2H2) IAA, (2H5) IPYA should allow more accurate quantitation of IAA in plant extracts.",

keywords = "deuterium‐labelled indole‐3‐pyruvic acid, indole‐3‐acetic acid, mass spectrometry",

author = "Jane Badenoch‐Jones and Summons, {Roger E.} and Letham, {David S.}",

year = "1983",

doi = "10.1002/jlcr.2580201203",

language = "English",

volume = "20",

pages = "1325--1330",

journal = "Journal of Labelled Compounds and Radiopharmaceuticals",

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T1 - Synthesis of (2H5) indole‐3‐pyruvic acid

AU - Badenoch‐Jones, Jane

AU - Summons, Roger E.

AU - Letham, David S.

PY - 1983

Y1 - 1983

N2 - A method for the synthesis of deuterium‐labelled tryptophan and its enzymatic conversion to indole‐3‐pyruvic acid ((2H5)IPYA) using amino acid oxidase is described. (2H5)IPYA can be used as an internal standard for the quantitation of IPYA by mass spectrometry in plant extracts and for estimating the breakdown of IPYA to indole‐3‐acetic acid (IAA) during sample extraction and purification. When used in conjunction with (2H2) IAA, (2H5) IPYA should allow more accurate quantitation of IAA in plant extracts.

AB - A method for the synthesis of deuterium‐labelled tryptophan and its enzymatic conversion to indole‐3‐pyruvic acid ((2H5)IPYA) using amino acid oxidase is described. (2H5)IPYA can be used as an internal standard for the quantitation of IPYA by mass spectrometry in plant extracts and for estimating the breakdown of IPYA to indole‐3‐acetic acid (IAA) during sample extraction and purification. When used in conjunction with (2H2) IAA, (2H5) IPYA should allow more accurate quantitation of IAA in plant extracts.

KW - deuterium‐labelled indole‐3‐pyruvic acid

KW - indole‐3‐acetic acid

KW - mass spectrometry

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DO - 10.1002/jlcr.2580201203

M3 - Article

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SP - 1325

EP - 1330

JO - Journal of Labelled Compounds and Radiopharmaceuticals

JF - Journal of Labelled Compounds and Radiopharmaceuticals

SN - 0362-4803

IS - 12