Systematic assessment of blood circulation time of functionalized upconversion nanoparticles in the chick embryo

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Abstract

Nanoparticle-based delivery of drugs and contrast agents holds great promise in cancer research, because of the increased delivery efficiency compared to 'free'™ drugs and dyes. A versatile platform to investigate nanotechnology is the chick embryo chorioallantoic membrane tumour model, due to its availability (easy, cheap) and accessibility (interventions, imaging). In our group, we developed this model using several tumour cell lines (e.g. breast cancer, colon cancer). In addition, we have synthesized in-house silica coated photoluminescent upconversion nanoparticles with several functional groups (COOH, NH2, PEG). In this work we will present the systematic assessment of their in vivo blood circulation times. To this end, we injected chick embryos grown ex ovo with the functionalized UCNPs and obtained a small amount of blood at several time points after injection to create blood smears The UCNP signal from the blood smears was quantified using a modified inverted microscope imaging set-up. The results of this systematic study are valuable to optimize biochemistry protocols and guide nanomedicine advancement in the versatile chick embryo tumour model.

Original languageEnglish
Title of host publicationMicro+Nano Materials, Devices, and Systems
EditorsBenjamin J. Eggleton, Stefano Palomba
Place of PublicationBellingham, WA
PublisherSPIE
Pages1-7
Number of pages7
ISBN (Print)9781628418903
DOIs
Publication statusPublished - Dec 2015
EventSPIE Micro+Nano Materials, Devices, and Applications Symposium - Sydney, Australia
Duration: 6 Dec 20159 Dec 2015

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume9668
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Other

OtherSPIE Micro+Nano Materials, Devices, and Applications Symposium
CountryAustralia
CitySydney
Period6/12/159/12/15

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Nadort, A., Liang, L., Grebenik, E., Guller, A., Lu, Y., Qian, Y., ... Zvyagin, A. (2015). Systematic assessment of blood circulation time of functionalized upconversion nanoparticles in the chick embryo. In B. J. Eggleton, & S. Palomba (Eds.), Micro+Nano Materials, Devices, and Systems (pp. 1-7). [96683Y] (Proceedings of SPIE; Vol. 9668). Bellingham, WA: SPIE. https://doi.org/10.1117/12.2202449