Abstract
Purpose: DNA-based aptamers have been proposed as a simultaneous active targeting agent and delivery vehicle for anticancer drugs. PHENSS is a platinum-based anticancer complex that reversibly binds to DNA. Previous research has shown that direct binding of platinum drugs to aptamers affects the aptamers' targeting ability. This study aims to attach PHENSS to DNA aptamers using high affinity carrier DNA segments based on bulge secondary DNA structures.
Methods: DNA melting experiments were conducted using UV-Vis spectrophotometry to observe intercalation of PHENSS in the bulge DNA carrier segments. Drug to DNA-aptamer binding was studied using circular dichroism spectrophotometry and 1D, 2D NOESY 1H-NMR. In vitro cytotoxicity studies were performed against a panel of human cancer cell lines.
Results: DNA melting experiments were conducted using UV-Vis spectrophotometry to observe intercalation of PHENSS in the bulge DNA carrier segments. Drug to DNA-aptamer binding was studied using circular dichroism spectrophotometry and 1D, 2D NOESY 1H-NMR. In vitro cytotoxicity studies were performed against a panel of human cancer cell lines.
Conclusion: Aptamers show potential as a simultaneous specific receptor targeted and anticancer delivery vehicles.
Methods: DNA melting experiments were conducted using UV-Vis spectrophotometry to observe intercalation of PHENSS in the bulge DNA carrier segments. Drug to DNA-aptamer binding was studied using circular dichroism spectrophotometry and 1D, 2D NOESY 1H-NMR. In vitro cytotoxicity studies were performed against a panel of human cancer cell lines.
Results: DNA melting experiments were conducted using UV-Vis spectrophotometry to observe intercalation of PHENSS in the bulge DNA carrier segments. Drug to DNA-aptamer binding was studied using circular dichroism spectrophotometry and 1D, 2D NOESY 1H-NMR. In vitro cytotoxicity studies were performed against a panel of human cancer cell lines.
Conclusion: Aptamers show potential as a simultaneous specific receptor targeted and anticancer delivery vehicles.
| Original language | English |
|---|---|
| Pages (from-to) | 42-43 |
| Number of pages | 2 |
| Journal | Asia-Pacific Journal of Clinical Oncology |
| Volume | 10 |
| Issue number | S7 |
| Publication status | Published - Nov 2014 |
| Externally published | Yes |