Targeted mass spectrometry of S100 proteins

Juan Martínez-Aguilar, Mark P. Molloy*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

4 Citations (Scopus)


The S100 protein family has attracted great interest in the field of biomarker research, and a growing number of studies reveal dysregulation of many of the 21 S100 protein isoforms in various human diseases. In cancer, S100 protein expression has been associated with tumor growth, progression, and response to treatment. Some S100 proteins are also considered candidate therapeutic targets. From an analytical perspective, multiplexed analysis of the family-wide S100 protein expression is challenging due to their relatively small size and high-sequence identity. Here we describe a mass spectrometry method using selected reaction monitoring which enables the targeted, multiplexed detection and quantitation of the entire S100 protein family in cell lines and tissue samples.

Original languageEnglish
Title of host publicationCalcium-binding proteins of the EF-hand superfamily
Subtitle of host publicationfrom basics to medical applications
EditorsClaus W. Heizmann
Place of PublicationNew York
PublisherHumana Press
Number of pages16
ISBN (Electronic)9781493990306
ISBN (Print)9781493990290
Publication statusPublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • S100 proteins
  • Ca²⁺-binding
  • EF-hand
  • Biomarkers
  • Quantitative proteomics
  • Targeted mass spectrometry
  • Selected reaction monitoring


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