Technical challenges of working with extracellular vesicles

Marcel I. Ramirez; Maria G. Amorim; Catarina Gadelha; Ivana Milic; Joshua A. Welsh; Vanessa M. Freitas; Muhammad Nawaz; Naveed Akbar; Yvonne Couch; Laura Makin; Fiona Cooke; Andre L. Vettore; Patricia X. Batista; Roberta Freezor; Julia A. Pezuk; Livia Rosa-Fernandes; Ana Claudia O. Carreira; Andrew Devitt; Laura Jacobs; Israel T. Silva; Gillian Coakley; Diana N. Nunes; Dave Carter; Giuseppe Palmisano; Emmanuel Dias-Neto

doi:10.1039/c7nr08360b

Technical challenges of working with extracellular vesicles

Marcel I. Ramirez, Maria G. Amorim, Catarina Gadelha, Ivana Milic, Joshua A. Welsh, Vanessa M. Freitas, Muhammad Nawaz, Naveed Akbar, Yvonne Couch, Laura Makin, Fiona Cooke, Andre L. Vettore, Patricia X. Batista, Roberta Freezor, Julia A. Pezuk, Livia Rosa-Fernandes, Ana Claudia O. Carreira, Andrew Devitt, Laura Jacobs, Israel T. SilvaGillian Coakley, Diana N. Nunes, Dave Carter, Giuseppe Palmisano, Emmanuel Dias-Neto

Research output: Contribution to journal › Review article › peer-review

491 Citations (Scopus)

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Abstract

Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

Original language	English
Pages (from-to)	881-906
Number of pages	26
Journal	Nanoscale
Volume	3
DOIs	https://doi.org/10.1039/c7nr08360b
Publication status	Published - 21 Jan 2018
Externally published	Yes

Bibliographical note

Copyright the Royal Society of Chemistry 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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10.1039/c7nr08360bLicence: CC BY

Publisher version (open access)Final published version, 1.23 MBLicence: CC BY

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Ramirez, M. I., Amorim, M. G., Gadelha, C., Milic, I., Welsh, J. A., Freitas, V. M., Nawaz, M., Akbar, N., Couch, Y., Makin, L., Cooke, F., Vettore, A. L., Batista, P. X., Freezor, R., Pezuk, J. A., Rosa-Fernandes, L., Carreira, A. C. O., Devitt, A., Jacobs, L., ... Dias-Neto, E. (2018). Technical challenges of working with extracellular vesicles. Nanoscale, 3, 881-906. https://doi.org/10.1039/c7nr08360b

@article{ddb3f739faa94ff286849553e9e0605c,

title = "Technical challenges of working with extracellular vesicles",

abstract = "Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.",

author = "Ramirez, \{Marcel I.\} and Amorim, \{Maria G.\} and Catarina Gadelha and Ivana Milic and Welsh, \{Joshua A.\} and Freitas, \{Vanessa M.\} and Muhammad Nawaz and Naveed Akbar and Yvonne Couch and Laura Makin and Fiona Cooke and Vettore, \{Andre L.\} and Batista, \{Patricia X.\} and Roberta Freezor and Pezuk, \{Julia A.\} and Livia Rosa-Fernandes and Carreira, \{Ana Claudia O.\} and Andrew Devitt and Laura Jacobs and Silva, \{Israel T.\} and Gillian Coakley and Nunes, \{Diana N.\} and Dave Carter and Giuseppe Palmisano and Emmanuel Dias-Neto",

note = "Copyright the Royal Society of Chemistry 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.",

year = "2018",

month = jan,

day = "21",

doi = "10.1039/c7nr08360b",

language = "English",

volume = "3",

pages = "881--906",

journal = "Nanoscale",

issn = "2040-3364",

publisher = "Royal Society of Chemistry",

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Ramirez, MI, Amorim, MG, Gadelha, C, Milic, I, Welsh, JA, Freitas, VM, Nawaz, M, Akbar, N, Couch, Y, Makin, L, Cooke, F, Vettore, AL, Batista, PX, Freezor, R, Pezuk, JA, Rosa-Fernandes, L, Carreira, ACO, Devitt, A, Jacobs, L, Silva, IT, Coakley, G, Nunes, DN, Carter, D, Palmisano, G & Dias-Neto, E 2018, 'Technical challenges of working with extracellular vesicles', Nanoscale, vol. 3, pp. 881-906. https://doi.org/10.1039/c7nr08360b

TY - JOUR

T1 - Technical challenges of working with extracellular vesicles

AU - Ramirez, Marcel I.

AU - Amorim, Maria G.

AU - Gadelha, Catarina

AU - Milic, Ivana

AU - Welsh, Joshua A.

AU - Freitas, Vanessa M.

AU - Nawaz, Muhammad

AU - Akbar, Naveed

AU - Couch, Yvonne

AU - Makin, Laura

AU - Cooke, Fiona

AU - Vettore, Andre L.

AU - Batista, Patricia X.

AU - Freezor, Roberta

AU - Pezuk, Julia A.

AU - Rosa-Fernandes, Livia

AU - Carreira, Ana Claudia O.

AU - Devitt, Andrew

AU - Jacobs, Laura

AU - Silva, Israel T.

AU - Coakley, Gillian

AU - Nunes, Diana N.

AU - Carter, Dave

AU - Palmisano, Giuseppe

AU - Dias-Neto, Emmanuel

N1 - Copyright the Royal Society of Chemistry 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2018/1/21

Y1 - 2018/1/21

N2 - Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

AB - Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

UR - https://www.scopus.com/pages/publications/85040917403

U2 - 10.1039/c7nr08360b

DO - 10.1039/c7nr08360b

M3 - Review article

C2 - 29265147

SN - 2040-3364

VL - 3

SP - 881

EP - 906

JO - Nanoscale

JF - Nanoscale

ER -

Technical challenges of working with extracellular vesicles

Abstract

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