Techniques for analysis of plant phenolic compounds

Ali Khoddami, Meredith A. Wilkes, Thomas H. Roberts*

*Corresponding author for this work

    Research output: Contribution to journalReview articlepeer-review

    860 Citations (Scopus)


    Phenolic compounds are well-known phytochemicals found in all plants. They consist of simple phenols, benzoic and cinnamic acid, coumarins, tannins, lignins, lignans and flavonoids. Substantial developments in research focused on the extraction, identification and quantification of phenolic compounds as medicinal and/or dietary molecules have occurred over the last 25 years. Organic solvent extraction is the main method used to extract phenolics. Chemical procedures are used to detect the presence of total phenolics, while spectrophotometric and chromatographic techniques are utilized to identify and quantify individual phenolic compounds. This review addresses the application of different methodologies utilized in the analysis of phenolic compounds in plant-based products, including recent technical developments in the quantification of phenolics.

    Original languageEnglish
    Pages (from-to)2328-2375
    Number of pages48
    Issue number2
    Publication statusPublished - Feb 2013


    • Food analysis
    • GC
    • HPLC
    • Phenolic compound
    • Phenolic extraction technique
    • Phenolic quantification method


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