The β-mannanase from 'Caldocellum saccharolyticum' is part of a multidomain enzyme

M. D. Gibbs, D. J. Saul, E. Luthi, P. L. Bergquist*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

71 Citations (Scopus)


The complete sequence of a β-mannanase gene from an anaerobic extreme thermophile was determined, and it shows that the expressed protein consists of two catalytic domains and two binding domains separated by spacer regions rich in proline and threonine residues. The amino-terminal catalytic domain has β-mannanase activity, and the carboxy-terminal domain acts as an endoglucanase. Neither domain shows homology with any other cellulase or hemicellulase sequence at the nucleic acid or protein level.

Original languageEnglish
Pages (from-to)3864-3867
Number of pages4
JournalApplied and Environmental Microbiology
Issue number12
Publication statusPublished - 1992
Externally publishedYes


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