The amyloid protein precursor of Alzheimer's disease is a mediator of the effects of nerve growth factor on neurite outgrowth

Elizabeth A. Milward; Roula Papadopoulos; Stephanie J. Fuller; Robert D. Moir; David Small; Konrad Beyreuther; Colin L. Masters

doi:10.1016/0896-6273(92)90228-6

The amyloid protein precursor of Alzheimer's disease is a mediator of the effects of nerve growth factor on neurite outgrowth

Elizabeth A. Milward, Roula Papadopoulos, Stephanie J. Fuller, Robert D. Moir, David Small, Konrad Beyreuther, Colin L. Masters

Research output: Contribution to journal › Article › peer-review

417 Citations (Scopus)

Abstract

The βA4 protein, the major component of the amyloid deposition characterizing Alzheimer's disease, derives from the amyloid protein precursor (APP), an integral membrane protein with soluble derivatives. The function of APP is unknown. Both soluble and membrane-associated human brain APP (10^-10 M) significantly increased (P less than 0.025) neurite length and branching in pheochromocytoma PC12 cells, but did not affect the number of neurites per cell. At higher concentrations, APP was cytotoxic, with a half-maximal concentration of 5 x 10^-9 M. Nerve growth factor (NGF) is known to affect APP expression in vivo and in vitro. Antibodies to APP specifically diminished the effects of NGF on neurite length and branching. Thus APP may act to mediate neurite outgrowth promotion by NGF.

Original language	English
Pages (from-to)	129-137
Number of pages	9
Journal	Neuron
Volume	9
Issue number	1
DOIs	https://doi.org/10.1016/0896-6273(92)90228-6
Publication status	Published - 1992
Externally published	Yes

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title = "The amyloid protein precursor of Alzheimer's disease is a mediator of the effects of nerve growth factor on neurite outgrowth",

abstract = "The βA4 protein, the major component of the amyloid deposition characterizing Alzheimer's disease, derives from the amyloid protein precursor (APP), an integral membrane protein with soluble derivatives. The function of APP is unknown. Both soluble and membrane-associated human brain APP (10-10 M) significantly increased (P less than 0.025) neurite length and branching in pheochromocytoma PC12 cells, but did not affect the number of neurites per cell. At higher concentrations, APP was cytotoxic, with a half-maximal concentration of 5 x 10-9 M. Nerve growth factor (NGF) is known to affect APP expression in vivo and in vitro. Antibodies to APP specifically diminished the effects of NGF on neurite length and branching. Thus APP may act to mediate neurite outgrowth promotion by NGF.",

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T1 - The amyloid protein precursor of Alzheimer's disease is a mediator of the effects of nerve growth factor on neurite outgrowth

AU - Milward, Elizabeth A.

AU - Papadopoulos, Roula

AU - Fuller, Stephanie J.

AU - Moir, Robert D.

AU - Small, David

AU - Beyreuther, Konrad

AU - Masters, Colin L.

PY - 1992

Y1 - 1992

N2 - The βA4 protein, the major component of the amyloid deposition characterizing Alzheimer's disease, derives from the amyloid protein precursor (APP), an integral membrane protein with soluble derivatives. The function of APP is unknown. Both soluble and membrane-associated human brain APP (10-10 M) significantly increased (P less than 0.025) neurite length and branching in pheochromocytoma PC12 cells, but did not affect the number of neurites per cell. At higher concentrations, APP was cytotoxic, with a half-maximal concentration of 5 x 10-9 M. Nerve growth factor (NGF) is known to affect APP expression in vivo and in vitro. Antibodies to APP specifically diminished the effects of NGF on neurite length and branching. Thus APP may act to mediate neurite outgrowth promotion by NGF.

AB - The βA4 protein, the major component of the amyloid deposition characterizing Alzheimer's disease, derives from the amyloid protein precursor (APP), an integral membrane protein with soluble derivatives. The function of APP is unknown. Both soluble and membrane-associated human brain APP (10-10 M) significantly increased (P less than 0.025) neurite length and branching in pheochromocytoma PC12 cells, but did not affect the number of neurites per cell. At higher concentrations, APP was cytotoxic, with a half-maximal concentration of 5 x 10-9 M. Nerve growth factor (NGF) is known to affect APP expression in vivo and in vitro. Antibodies to APP specifically diminished the effects of NGF on neurite length and branching. Thus APP may act to mediate neurite outgrowth promotion by NGF.

U2 - 10.1016/0896-6273(92)90228-6

DO - 10.1016/0896-6273(92)90228-6

M3 - Article

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VL - 9

SP - 129

EP - 137

JO - Neuron

JF - Neuron

IS - 1

ER -

The amyloid protein precursor of Alzheimer's disease is a mediator of the effects of nerve growth factor on neurite outgrowth

Abstract

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