The apoptotic activity of one VLC fraction of the sponge Petrosia tuberosa on human cervical cells and the subsequent isolation of a bioactive polyacetylene

Avin Ramanjooloo, Girish Beedessee, Deepak Arya, Rob W M VanSoest, Thierry Cresteil, Daniel E P Marie*

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

As part of our ongoing studies on bioactive natural products from marine sponges, we investigated the cytotoxic potential of extracts from the new sponge Petrosia tuberosa sampled from Mauritius waters. Bioguided fractionation of the ethyl acetate extract by vacuum liquid chromatography (VLC) revealed two fractions, namely VLC (6-9) and (13-17) showing cell deaths of 86 ± 1% and 88 ± 4%, respectively, at 50 μg/mL on HeLa cells. At 10 μg/mL, only VLC (13-17) displayed a significant cell death (56 ± 7%) compared with VLC (6-9) (8 ± 1%). The cytotoxic activity of VLC (13-17) was also determined on nine other human cancer cell lines. Clonogenic assay, mitochondrial membrane potential change, DNA fragmentation and microscopic analysis of fraction VLC (13-17) revealed distinct features of apoptosis on HeLa cells. Further fractionation and purification of this fraction by chromatographic techniques resulted in isolation of one known secondary metabolite, petrosynol. Its structure was determined by 1H and 13C-NMR analyses.

Original languageEnglish
Pages (from-to)635-638
Number of pages4
JournalNatural Product Communications
Volume8
Issue number5
Publication statusPublished - 2013

Keywords

  • Clonogenic assay
  • Cytotoxic
  • DNA fragmentation
  • Mitochondrial
  • MTT
  • VLC

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