Abstract
Mass spectrometry has significantly contributed to the advancement of glycoprotein research. In combination with conventional glycan purification strategies, high throughput studies become practical, yielding a significant amount of detail regarding the glycoproteome. In this chapter, we provide an overview of commonly employed glycoprotein enrichment strategies which exploit the unique chemical features of glycans for their purification, and introduce the basic concepts of mass spectrometric methods as they apply to glycan identification and characterization. Landmark studies in the enrichment workflows and mass spectrometric analysis of glycans are summarized, as well as the major challenges that face glycoproteomic research.
Original language | English |
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Title of host publication | Functional and Structural Proteomics of Glycoproteins |
Editors | Raymond J. Owens, Joanne E. Nettleship |
Place of Publication | Dordrecht; New York |
Publisher | Springer, Springer Nature |
Pages | 103-125 |
Number of pages | 23 |
ISBN (Electronic) | 9789048193554 |
ISBN (Print) | 9789048193547, 9048193540 |
DOIs | |
Publication status | Published - 2011 |
Externally published | Yes |
Keywords
- Glycan enrichment techniques
- Glycopeptides
- Glycoproteomics
- High throughput
- Mass spectrometry