The application of high throughput mass spectrometry to the analysis of glycoproteins

Sasha Singh, Morten Thaysen Andersen, Judith Jebanathirajah Steen*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Citation (Scopus)


Mass spectrometry has significantly contributed to the advancement of glycoprotein research. In combination with conventional glycan purification strategies, high throughput studies become practical, yielding a significant amount of detail regarding the glycoproteome. In this chapter, we provide an overview of commonly employed glycoprotein enrichment strategies which exploit the unique chemical features of glycans for their purification, and introduce the basic concepts of mass spectrometric methods as they apply to glycan identification and characterization. Landmark studies in the enrichment workflows and mass spectrometric analysis of glycans are summarized, as well as the major challenges that face glycoproteomic research.

Original languageEnglish
Title of host publicationFunctional and Structural Proteomics of Glycoproteins
EditorsRaymond J. Owens, Joanne E. Nettleship
Place of PublicationDordrecht; New York
PublisherSpringer, Springer Nature
Number of pages23
ISBN (Electronic)9789048193554
ISBN (Print)9789048193547, 9048193540
Publication statusPublished - 2011
Externally publishedYes


  • Glycan enrichment techniques
  • Glycopeptides
  • Glycoproteomics
  • High throughput
  • Mass spectrometry


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