The application of high throughput mass spectrometry to the analysis of glycoproteins

Sasha Singh; Morten Thaysen Andersen; Judith Jebanathirajah Steen

doi:10.1007/978-90-481-9355-4-5

The application of high throughput mass spectrometry to the analysis of glycoproteins

Sasha Singh, Morten Thaysen Andersen, Judith Jebanathirajah Steen^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Citation (Scopus)

Abstract

Mass spectrometry has significantly contributed to the advancement of glycoprotein research. In combination with conventional glycan purification strategies, high throughput studies become practical, yielding a significant amount of detail regarding the glycoproteome. In this chapter, we provide an overview of commonly employed glycoprotein enrichment strategies which exploit the unique chemical features of glycans for their purification, and introduce the basic concepts of mass spectrometric methods as they apply to glycan identification and characterization. Landmark studies in the enrichment workflows and mass spectrometric analysis of glycans are summarized, as well as the major challenges that face glycoproteomic research.

Original language	English
Title of host publication	Functional and Structural Proteomics of Glycoproteins
Editors	Raymond J. Owens, Joanne E. Nettleship
Place of Publication	Dordrecht; New York
Publisher	Springer, Springer Nature
Pages	103-125
Number of pages	23
ISBN (Electronic)	9789048193554
ISBN (Print)	9789048193547, 9048193540
DOIs	https://doi.org/10.1007/978-90-481-9355-4-5
Publication status	Published - 2011
Externally published	Yes

Keywords

Glycan enrichment techniques
Glycopeptides
Glycoproteomics
High throughput
Mass spectrometry

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10.1007/978-90-481-9355-4-5

Cite this

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title = "The application of high throughput mass spectrometry to the analysis of glycoproteins",

abstract = "Mass spectrometry has significantly contributed to the advancement of glycoprotein research. In combination with conventional glycan purification strategies, high throughput studies become practical, yielding a significant amount of detail regarding the glycoproteome. In this chapter, we provide an overview of commonly employed glycoprotein enrichment strategies which exploit the unique chemical features of glycans for their purification, and introduce the basic concepts of mass spectrometric methods as they apply to glycan identification and characterization. Landmark studies in the enrichment workflows and mass spectrometric analysis of glycans are summarized, as well as the major challenges that face glycoproteomic research.",

keywords = "Glycan enrichment techniques, Glycopeptides, Glycoproteomics, High throughput, Mass spectrometry",

author = "Sasha Singh and Andersen, \{Morten Thaysen\} and Steen, \{Judith Jebanathirajah\}",

year = "2011",

doi = "10.1007/978-90-481-9355-4-5",

language = "English",

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pages = "103--125",

editor = "Owens, \{Raymond J.\} and Nettleship, \{Joanne E.\}",

booktitle = "Functional and Structural Proteomics of Glycoproteins",

publisher = "Springer, Springer Nature",

address = "United States",

}

The application of high throughput mass spectrometry to the analysis of glycoproteins. / Singh, Sasha; Andersen, Morten Thaysen; Steen, Judith Jebanathirajah.
Functional and Structural Proteomics of Glycoproteins. ed. / Raymond J. Owens; Joanne E. Nettleship. Dordrecht; New York: Springer, Springer Nature, 2011. p. 103-125.

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - The application of high throughput mass spectrometry to the analysis of glycoproteins

AU - Singh, Sasha

AU - Andersen, Morten Thaysen

AU - Steen, Judith Jebanathirajah

PY - 2011

Y1 - 2011

N2 - Mass spectrometry has significantly contributed to the advancement of glycoprotein research. In combination with conventional glycan purification strategies, high throughput studies become practical, yielding a significant amount of detail regarding the glycoproteome. In this chapter, we provide an overview of commonly employed glycoprotein enrichment strategies which exploit the unique chemical features of glycans for their purification, and introduce the basic concepts of mass spectrometric methods as they apply to glycan identification and characterization. Landmark studies in the enrichment workflows and mass spectrometric analysis of glycans are summarized, as well as the major challenges that face glycoproteomic research.

AB - Mass spectrometry has significantly contributed to the advancement of glycoprotein research. In combination with conventional glycan purification strategies, high throughput studies become practical, yielding a significant amount of detail regarding the glycoproteome. In this chapter, we provide an overview of commonly employed glycoprotein enrichment strategies which exploit the unique chemical features of glycans for their purification, and introduce the basic concepts of mass spectrometric methods as they apply to glycan identification and characterization. Landmark studies in the enrichment workflows and mass spectrometric analysis of glycans are summarized, as well as the major challenges that face glycoproteomic research.

KW - Glycan enrichment techniques

KW - Glycopeptides

KW - Glycoproteomics

KW - High throughput

KW - Mass spectrometry

UR - https://www.scopus.com/pages/publications/84919662529

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AN - SCOPUS:84919662529

SN - 9789048193547

SN - 9048193540

SP - 103

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BT - Functional and Structural Proteomics of Glycoproteins

A2 - Owens, Raymond J.

A2 - Nettleship, Joanne E.

PB - Springer, Springer Nature

CY - Dordrecht; New York

ER -

The application of high throughput mass spectrometry to the analysis of glycoproteins

Abstract

Keywords

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