The Art of validating quantitative proteomics data

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Western blotting as an orthogonal validation tool for quantitative proteomics data has rapidly become a de facto requirement for publication. In this viewpoint article, the pros and cons of western blotting as a validation approach are discussed, using examples from our own published work, and how to best apply it to improve the quality of data published is outlined. Further, suggestions and guidelines for some other experimental approaches are provided, which can be used for validation of quantitative proteomics data in addition to, or in place of, western blotting.
LanguageEnglish
Article number1800222
Pages1-6
Number of pages6
JournalProteomics
Volume18
Issue number23
DOIs
Publication statusPublished - Dec 2018

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Art
Proteomics
Western Blotting
Publications
Guidelines

Keywords

  • data quality
  • data validation
  • false discovery rate
  • shotgun proteomics

Cite this

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abstract = "Western blotting as an orthogonal validation tool for quantitative proteomics data has rapidly become a de facto requirement for publication. In this viewpoint article, the pros and cons of western blotting as a validation approach are discussed, using examples from our own published work, and how to best apply it to improve the quality of data published is outlined. Further, suggestions and guidelines for some other experimental approaches are provided, which can be used for validation of quantitative proteomics data in addition to, or in place of, western blotting.",
keywords = "data quality, data validation, false discovery rate, shotgun proteomics",
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The Art of validating quantitative proteomics data. / Handler, David C.; Pascovici, Dana; Mirzaei, Mehdi; Gupta, Vivek; Salekdeh, Ghassem Hosseini; Haynes, Paul A.

In: Proteomics, Vol. 18, No. 23, 1800222, 12.2018, p. 1-6.

Research output: Contribution to journalArticleResearchpeer-review

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