The association between personal sun exposure, serum vitamin D and global methylation in human lymphocytes in a population of healthy adults in South Australia

Visalini Nair-Shalliker; Varinderpal Dhillon; Mark Clements; Bruce K. Armstrong; Michael Fenech

doi:10.1016/j.mrfmmm.2014.04.001

The association between personal sun exposure, serum vitamin D and global methylation in human lymphocytes in a population of healthy adults in South Australia

Visalini Nair-Shalliker^*, Varinderpal Dhillon, Mark Clements, Bruce K. Armstrong, Michael Fenech

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Background: There is a positive association between solar UVexposure and micronucleus frequency in peripheral blood lymphocytes (PBL) andthis association may be stronger when serum vitamin D (25(OH)D) levels areinsufficient (<50 nmol/L). Micronucleus formation can result from globalhypomethylation of DNA repeat sequences. The aim of this analysis was toevaluate the relationship between solar UV exposure and methylation pattern inLINE-1 repetitive elements in PBL DNA and to see if serum 25(OH)D levels modifyit.

Method: Personal solar UV exposure was estimated from hours of outdoor exposure over 6 weeks recalled at the time of blood collection in 208 male and female participants living in South Australia. Methylation in LINE-1 repetitive elements was assessed in PBL using pyrosequencing.

Results: Methylation in LINE-1 decreased with increasing solar UV exposure (% decrease = 0.5% per doubling of sUV; 95%CI: −0.7 to −0.2 p_value= 0.00003). Although there was no correlation between LINE-1 methylation and micronucleus frequency, there was a 4.3% increase (95%CI: 0.6–8.1 p-value = 0.02) in nucleoplasmic bridges and a 4.3% increase in necrosis (CI: 1.9–6.8 p-value = 0.0005) for every 1% increase in LINE-1 methylation. Serum 25(OH)D was not associated with DNA methylation; or did it modify the association of solar UV with DNA methylation.

Conclusion: Exposure to solar UV radiation may reduce DNA methylation in circulating lymphocytes. This association does not appear to be influenced or mediated by vitamin D status.

Original language	English
Pages (from-to)	6-10
Number of pages	5
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	765
DOIs	https://doi.org/10.1016/j.mrfmmm.2014.04.001
Publication status	Published - Jul 2014
Externally published	Yes

Keywords

Solar UV exposure
Vitamin D
Global methylation
LINE-1 repetitive elements
DNA damage

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10.1016/j.mrfmmm.2014.04.001

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Nair-Shalliker, V., Dhillon, V., Clements, M., Armstrong, B. K., & Fenech, M. (2014). The association between personal sun exposure, serum vitamin D and global methylation in human lymphocytes in a population of healthy adults in South Australia. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 765, 6-10. https://doi.org/10.1016/j.mrfmmm.2014.04.001

@article{77ba154b4e3d476197d918d52d566f8b,

title = "The association between personal sun exposure, serum vitamin D and global methylation in human lymphocytes in a population of healthy adults in South Australia",

abstract = "Background: There is a positive association between solar UVexposure and micronucleus frequency in peripheral blood lymphocytes (PBL) andthis association may be stronger when serum vitamin D (25(OH)D) levels areinsufficient (<50 nmol/L). Micronucleus formation can result from globalhypomethylation of DNA repeat sequences. The aim of this analysis was toevaluate the relationship between solar UV exposure and methylation pattern inLINE-1 repetitive elements in PBL DNA and to see if serum 25(OH)D levels modifyit.Method: Personal solar UV exposure was estimated from hours of outdoor exposure over 6 weeks recalled at the time of blood collection in 208 male and female participants living in South Australia. Methylation in LINE-1 repetitive elements was assessed in PBL using pyrosequencing.Results: Methylation in LINE-1 decreased with increasing solar UV exposure (% decrease = 0.5% per doubling of sUV; 95%CI: −0.7 to −0.2 pvalue = 0.00003). Although there was no correlation between LINE-1 methylation and micronucleus frequency, there was a 4.3% increase (95%CI: 0.6–8.1 p-value = 0.02) in nucleoplasmic bridges and a 4.3% increase in necrosis (CI: 1.9–6.8 p-value = 0.0005) for every 1% increase in LINE-1 methylation. Serum 25(OH)D was not associated with DNA methylation; or did it modify the association of solar UV with DNA methylation.Conclusion: Exposure to solar UV radiation may reduce DNA methylation in circulating lymphocytes. This association does not appear to be influenced or mediated by vitamin D status.",

keywords = "Solar UV exposure, Vitamin D, Global methylation, LINE-1 repetitive elements, DNA damage",

author = "Visalini Nair-Shalliker and Varinderpal Dhillon and Mark Clements and Armstrong, {Bruce K.} and Michael Fenech",

year = "2014",

month = jul,

doi = "10.1016/j.mrfmmm.2014.04.001",

language = "English",

volume = "765",

pages = "6--10",

journal = "Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis",

issn = "0027-5107",

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Nair-Shalliker, V, Dhillon, V, Clements, M, Armstrong, BK & Fenech, M 2014, 'The association between personal sun exposure, serum vitamin D and global methylation in human lymphocytes in a population of healthy adults in South Australia', Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, vol. 765, pp. 6-10. https://doi.org/10.1016/j.mrfmmm.2014.04.001

The association between personal sun exposure, serum vitamin D and global methylation in human lymphocytes in a population of healthy adults in South Australia. / Nair-Shalliker, Visalini; Dhillon, Varinderpal; Clements, Mark et al.
In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 765, 07.2014, p. 6-10.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The association between personal sun exposure, serum vitamin D and global methylation in human lymphocytes in a population of healthy adults in South Australia

AU - Nair-Shalliker, Visalini

AU - Dhillon, Varinderpal

AU - Clements, Mark

AU - Armstrong, Bruce K.

AU - Fenech, Michael

PY - 2014/7

Y1 - 2014/7

N2 - Background: There is a positive association between solar UVexposure and micronucleus frequency in peripheral blood lymphocytes (PBL) andthis association may be stronger when serum vitamin D (25(OH)D) levels areinsufficient (<50 nmol/L). Micronucleus formation can result from globalhypomethylation of DNA repeat sequences. The aim of this analysis was toevaluate the relationship between solar UV exposure and methylation pattern inLINE-1 repetitive elements in PBL DNA and to see if serum 25(OH)D levels modifyit.Method: Personal solar UV exposure was estimated from hours of outdoor exposure over 6 weeks recalled at the time of blood collection in 208 male and female participants living in South Australia. Methylation in LINE-1 repetitive elements was assessed in PBL using pyrosequencing.Results: Methylation in LINE-1 decreased with increasing solar UV exposure (% decrease = 0.5% per doubling of sUV; 95%CI: −0.7 to −0.2 pvalue = 0.00003). Although there was no correlation between LINE-1 methylation and micronucleus frequency, there was a 4.3% increase (95%CI: 0.6–8.1 p-value = 0.02) in nucleoplasmic bridges and a 4.3% increase in necrosis (CI: 1.9–6.8 p-value = 0.0005) for every 1% increase in LINE-1 methylation. Serum 25(OH)D was not associated with DNA methylation; or did it modify the association of solar UV with DNA methylation.Conclusion: Exposure to solar UV radiation may reduce DNA methylation in circulating lymphocytes. This association does not appear to be influenced or mediated by vitamin D status.

AB - Background: There is a positive association between solar UVexposure and micronucleus frequency in peripheral blood lymphocytes (PBL) andthis association may be stronger when serum vitamin D (25(OH)D) levels areinsufficient (<50 nmol/L). Micronucleus formation can result from globalhypomethylation of DNA repeat sequences. The aim of this analysis was toevaluate the relationship between solar UV exposure and methylation pattern inLINE-1 repetitive elements in PBL DNA and to see if serum 25(OH)D levels modifyit.Method: Personal solar UV exposure was estimated from hours of outdoor exposure over 6 weeks recalled at the time of blood collection in 208 male and female participants living in South Australia. Methylation in LINE-1 repetitive elements was assessed in PBL using pyrosequencing.Results: Methylation in LINE-1 decreased with increasing solar UV exposure (% decrease = 0.5% per doubling of sUV; 95%CI: −0.7 to −0.2 pvalue = 0.00003). Although there was no correlation between LINE-1 methylation and micronucleus frequency, there was a 4.3% increase (95%CI: 0.6–8.1 p-value = 0.02) in nucleoplasmic bridges and a 4.3% increase in necrosis (CI: 1.9–6.8 p-value = 0.0005) for every 1% increase in LINE-1 methylation. Serum 25(OH)D was not associated with DNA methylation; or did it modify the association of solar UV with DNA methylation.Conclusion: Exposure to solar UV radiation may reduce DNA methylation in circulating lymphocytes. This association does not appear to be influenced or mediated by vitamin D status.

KW - Solar UV exposure

KW - Vitamin D

KW - Global methylation

KW - LINE-1 repetitive elements

KW - DNA damage

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UR - http://purl.org/au-research/grants/nhmrc/464895

U2 - 10.1016/j.mrfmmm.2014.04.001

DO - 10.1016/j.mrfmmm.2014.04.001

M3 - Article

C2 - 24727138

AN - SCOPUS:84899718432

SN - 0027-5107

VL - 765

SP - 6

EP - 10

JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

ER -

The association between personal sun exposure, serum vitamin D and global methylation in human lymphocytes in a population of healthy adults in South Australia

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Keywords

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