The chlorophyll-protein complexes of Prochloron sp. (Prochlorophyta)

Roger G. Hiller; A. W D Larkum

doi:10.1016/0005-2728(85)90086-6

The chlorophyll-protein complexes of Prochloron sp. (Prochlorophyta)

Roger G. Hiller^*, A. W D Larkum

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Chlorophyll-protein complexes have been isolated from Prochloron sp. by SDS-polyacrylamide gel electrophoresis and SDS-sucrose-gradient centrifugation. Complexes associated with Photosystem I have significant amounts of chlorophyll b and a principle polypeptide of 70 kDa. The largest Photosystem I complex had an M_r of more than 300 000 kDa, a chlorophyll a b ratio of 3.8 and a chlorophyll/P-700 ratio of approx. 100. Complexes enriched in chlorophyll b showed reduced electrophoretic mobility compared to spinach LHCP3, a higher Chl a b ratio (approx. 2.4) and had a principle polypeptide of 34 kDa. Neither the 34 kDa or any other polypeptide showed cross-reactivity with antibodies to spinach light-harvesting chlorophyll a b protein in a Western blot test.

Original language	English
Pages (from-to)	107-115
Number of pages	9
Journal	BBA - Bioenergetics
Volume	806
Issue number	1
DOIs	https://doi.org/10.1016/0005-2728(85)90086-6
Publication status	Published - 23 Jan 1985

Keywords

(Prochloron, Prochlorophyta)
Chlorophyll b
Chlorophyll-protein complex
Light-harvesting complex
P-700
Photosystem I

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10.1016/0005-2728(85)90086-6

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title = "The chlorophyll-protein complexes of Prochloron sp. (Prochlorophyta)",

abstract = "Chlorophyll-protein complexes have been isolated from Prochloron sp. by SDS-polyacrylamide gel electrophoresis and SDS-sucrose-gradient centrifugation. Complexes associated with Photosystem I have significant amounts of chlorophyll b and a principle polypeptide of 70 kDa. The largest Photosystem I complex had an Mr of more than 300 000 kDa, a chlorophyll a b ratio of 3.8 and a chlorophyll/P-700 ratio of approx. 100. Complexes enriched in chlorophyll b showed reduced electrophoretic mobility compared to spinach LHCP3, a higher Chl a b ratio (approx. 2.4) and had a principle polypeptide of 34 kDa. Neither the 34 kDa or any other polypeptide showed cross-reactivity with antibodies to spinach light-harvesting chlorophyll a b protein in a Western blot test.",

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T1 - The chlorophyll-protein complexes of Prochloron sp. (Prochlorophyta)

AU - Hiller, Roger G.

AU - Larkum, A. W D

PY - 1985/1/23

Y1 - 1985/1/23

N2 - Chlorophyll-protein complexes have been isolated from Prochloron sp. by SDS-polyacrylamide gel electrophoresis and SDS-sucrose-gradient centrifugation. Complexes associated with Photosystem I have significant amounts of chlorophyll b and a principle polypeptide of 70 kDa. The largest Photosystem I complex had an Mr of more than 300 000 kDa, a chlorophyll a b ratio of 3.8 and a chlorophyll/P-700 ratio of approx. 100. Complexes enriched in chlorophyll b showed reduced electrophoretic mobility compared to spinach LHCP3, a higher Chl a b ratio (approx. 2.4) and had a principle polypeptide of 34 kDa. Neither the 34 kDa or any other polypeptide showed cross-reactivity with antibodies to spinach light-harvesting chlorophyll a b protein in a Western blot test.

AB - Chlorophyll-protein complexes have been isolated from Prochloron sp. by SDS-polyacrylamide gel electrophoresis and SDS-sucrose-gradient centrifugation. Complexes associated with Photosystem I have significant amounts of chlorophyll b and a principle polypeptide of 70 kDa. The largest Photosystem I complex had an Mr of more than 300 000 kDa, a chlorophyll a b ratio of 3.8 and a chlorophyll/P-700 ratio of approx. 100. Complexes enriched in chlorophyll b showed reduced electrophoretic mobility compared to spinach LHCP3, a higher Chl a b ratio (approx. 2.4) and had a principle polypeptide of 34 kDa. Neither the 34 kDa or any other polypeptide showed cross-reactivity with antibodies to spinach light-harvesting chlorophyll a b protein in a Western blot test.

KW - (Prochloron, Prochlorophyta)

KW - Chlorophyll b

KW - Chlorophyll-protein complex

KW - Light-harvesting complex

KW - P-700

KW - Photosystem I

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The chlorophyll-protein complexes of Prochloron sp. (Prochlorophyta)

Abstract

Keywords

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