The contrasting microRNA content of a drought tolerant and a drought susceptible wheat cultivar

Behnam Bakhshi, Ehsan Mohseni Fard, Javad Gharechahi, Mahdieh Safarzadeh, Nava Nikpay, Reza Fotovat, Mohammad Reza Azimi, Ghasem Hosseini Salekdeh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Drought stress represents one of the most common stresses affecting the productivity of crop plants. A rather recently discovered component of the plant response to drought is the cellular population of microRNAs. Here, the microRNA content was revealed of two bread wheat cultivars contrasting strongly with respect to the ability to withstand drought stress. A total of 1813 miRNAs was identified, grouped into 106 families. Some 104 of these miRNAs were predicted to match 212 novel miRNA precursors. In the drought tolerant cultivar (SM), 105 (33 known and 72 novel) miRNAs were altered in abundance by the imposition of drought stress, while the equivalent number in the more sensitive cultivar (SW) was 51 (20 and 31). An in silico analysis predicted that these miRNAs target at least 1959 genes in SM and 1111 in SW, suggesting their broad contribution to the drought stress response. Among the target genes were several known stress-related genes, encoding, for example, superoxide dismutase, various MYB transcription factors, various ABA signaling proteins and various MADS-box transcription factors. In many cases, the more susceptible cultivar SW behaved in a contrasting manner. The suggestion is that miRNAs represent an important aspect of the drought stress response, post-transcriptionally regulating a range of stress-related genes.

Original languageEnglish
Pages (from-to)35-43
Number of pages9
JournalJournal of Plant Physiology
Volume216
DOIs
Publication statusPublished - Sept 2017
Externally publishedYes

Keywords

  • miRNA
  • Target genes
  • Next-generation sequencing
  • Drought stress
  • Post-transcriptional gene regulation

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