The effect of dexamethasone on transferrin secretion by cultured fetal rat hepatocytes

M. Kraemer, J. Vassy, V. Brighton, Stephanie Fuller, G. Yeoh

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Cultured fetal rat hepatocytes derived from 12, 15 and 19-day gestation rats are capable of secreting transferrin. When dexamethasone is added to the medium an increased secretion rate is observed. The changes in secretion rates in control as well as dexamethasone-treated cells during culture have been shown to correlate with the level of mRNA coding for transferrin. Immunocytochemical experiments show that initially all hepatocytes contain transferrin which is localized in the lumina of the perinuclear space, rough endoplasmic reticulum and in the saccules and vesicles of the Golgi apparatus. During culture, particularly in control cells, the intensity of labelling varies from cell to cell. In addition, adjacent cells are observed to label more intensely in different intracellular organelles.
Original languageEnglish
Pages (from-to)52-59
Number of pages8
JournalEuropean Journal of Cell Biology
Volume42
Issue number1
Publication statusPublished - 1986
Externally publishedYes

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