The effect of nociceptin on Ca2+ channel current and intracellular Ca2+ in the SH-SY5Y human neuroblastoma cell line

Mark Connor, Alison Yeo, Graeme Henderson*

*Corresponding author for this work

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

The human neuroblastoma cell line SH-SY5Y expresses the 'orphan' opioid receptor (ORL1). We have demonstrated that nociceptin, the putative endogenous ligand for ORL1, produces a concentration-dependent inhibition of the N-type calcium channel current in these cells (IC50 42 nM). In addition, in the presence of carbachol, nociceptin increased the intracellular concentration of Ca2+ (EC50 60 nM). Both effects of nociceptin were blocked by pertussis toxin pretreatment but not by the opioid antagonists CTAP (1 μM), naltrindole (1 μM) and naloxone (10 μM).

Original languageEnglish
Pages (from-to)205-207
Number of pages3
JournalBritish Journal of Pharmacology
Volume118
Issue number2
Publication statusPublished - 1996
Externally publishedYes

Keywords

  • Calcium currents
  • Calcium release
  • Nociceptin
  • ORL1
  • Orphanin FQ
  • SH-SY5Y cells

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