Abstract
The human neuroblastoma cell line SH-SY5Y expresses the 'orphan' opioid receptor (ORL1). We have demonstrated that nociceptin, the putative endogenous ligand for ORL1, produces a concentration-dependent inhibition of the N-type calcium channel current in these cells (IC50 42 nM). In addition, in the presence of carbachol, nociceptin increased the intracellular concentration of Ca2+ (EC50 60 nM). Both effects of nociceptin were blocked by pertussis toxin pretreatment but not by the opioid antagonists CTAP (1 μM), naltrindole (1 μM) and naloxone (10 μM).
| Original language | English |
|---|---|
| Pages (from-to) | 205-207 |
| Number of pages | 3 |
| Journal | British Journal of Pharmacology |
| Volume | 118 |
| Issue number | 2 |
| Publication status | Published - 1996 |
| Externally published | Yes |
Keywords
- Calcium currents
- Calcium release
- Nociceptin
- ORL1
- Orphanin FQ
- SH-SY5Y cells