The effects of α-tocopherol on site-specific lipid peroxidation induced by iron in charged micelles

Kenji Fukuzawa*, Katsuya Kishikawa, Toyohiro Tadokoro, Akira Tokumura, Hiroaki Tsukatani, Janusz M. Gebicki

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

α-Tocopherol inhibited H2O2-Fe2+-induced lipid peroxidation of linoleic acid (LA) by scavenging OH radicals in tetradecyltrimethylammonium bromide (TTAB) micelles. The inhibiting ability of α-tocopherol was much greater than that of OH-radical scavengers mannitol and t-butanol. In contrast, α-tocopherol enhanced linoleic acid hydroperoxide (LOOH)Fe2+-induced lipid peroxidation through regeneration of Fe2+ in sodium dodecyl sulfate (SDS) micelles containing LA. α-Tocopherol was oxidized by Fenton's reagent (FeSO4 + H2O2) at a higher rate in SDS micelles than in TTAB micelles. The likely oxidants were OH radicals in the former and Fe3+ in the latter. Both reagents formed in the Fenton reaction. Ferrous ion catalyzed in a dose-dependent manner the decomposition of LOOH and conjugated diene compounds in SDS but not in TTAB micelles. α-Tocopherol and Fe3+ individually had no effect on the decomposition of LOOH, but together were quite effective. The rate of the decomposition was a function of the concentration of α-tocopherol. The mechanism of "site-specific" antioxidant action of α-tocopherol in charged micelles is discussed.

Original languageEnglish
Pages (from-to)153-160
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume260
Issue number1
DOIs
Publication statusPublished - 1988

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