The effects of intravaginal oestradiol - 3: 17β on the cell structure of the vaginal epithelium of the ovariectomized mouse

Irina Pollard, L. Martin, C. D. Shorey

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The ultrastructure of the vaginal epithelium of the ovariectomized mouse, was studied after oestradiol treatment. Tissue for electron microscopy was fixed at various times after injection in 2% osmium tetroxide and embedded in "Araldite". The earliest change seen in the vaginal epithelium after the intravaginal application of oestradiol occurred in nucleolar structure. In untreated animals nucleoli were small and of relatively uniform texture. After local application of oestradiol they increased in size and changed in appearance due to the development of large deeply staining intranucleolar bodies. The response became evident 1 hr after application and reached a maximum at 2-5 hr. Nucleoli from treated animals retained this structure up to the latest time of study (20 hr). Changes were also observed in mitochondrial number, cell and nuclear size, but these changes were small and inconsistent and occurred only at the later times. The significance of these results is discussed in relation to the action of oestradiol.

LanguageEnglish
Pages805-823
Number of pages19
JournalSteroids
Volume8
Issue number6
Publication statusPublished - Dec 1966
Externally publishedYes

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Estradiol
Epithelium
Animals
Cell Nucleus Size
Osmium Tetroxide
Animal Structures
Time and Motion Studies
Electron microscopy
Electron Microscopy
Textures
Tissue
Staining and Labeling
Injections
Therapeutics

Cite this

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title = "The effects of intravaginal oestradiol - 3: 17β on the cell structure of the vaginal epithelium of the ovariectomized mouse",
abstract = "The ultrastructure of the vaginal epithelium of the ovariectomized mouse, was studied after oestradiol treatment. Tissue for electron microscopy was fixed at various times after injection in 2{\%} osmium tetroxide and embedded in {"}Araldite{"}. The earliest change seen in the vaginal epithelium after the intravaginal application of oestradiol occurred in nucleolar structure. In untreated animals nucleoli were small and of relatively uniform texture. After local application of oestradiol they increased in size and changed in appearance due to the development of large deeply staining intranucleolar bodies. The response became evident 1 hr after application and reached a maximum at 2-5 hr. Nucleoli from treated animals retained this structure up to the latest time of study (20 hr). Changes were also observed in mitochondrial number, cell and nuclear size, but these changes were small and inconsistent and occurred only at the later times. The significance of these results is discussed in relation to the action of oestradiol.",
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The effects of intravaginal oestradiol - 3 : 17β on the cell structure of the vaginal epithelium of the ovariectomized mouse. / Pollard, Irina; Martin, L.; Shorey, C. D.

In: Steroids, Vol. 8, No. 6, 12.1966, p. 805-823.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The effects of intravaginal oestradiol - 3

T2 - Steroids

AU - Pollard, Irina

AU - Martin, L.

AU - Shorey, C. D.

PY - 1966/12

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N2 - The ultrastructure of the vaginal epithelium of the ovariectomized mouse, was studied after oestradiol treatment. Tissue for electron microscopy was fixed at various times after injection in 2% osmium tetroxide and embedded in "Araldite". The earliest change seen in the vaginal epithelium after the intravaginal application of oestradiol occurred in nucleolar structure. In untreated animals nucleoli were small and of relatively uniform texture. After local application of oestradiol they increased in size and changed in appearance due to the development of large deeply staining intranucleolar bodies. The response became evident 1 hr after application and reached a maximum at 2-5 hr. Nucleoli from treated animals retained this structure up to the latest time of study (20 hr). Changes were also observed in mitochondrial number, cell and nuclear size, but these changes were small and inconsistent and occurred only at the later times. The significance of these results is discussed in relation to the action of oestradiol.

AB - The ultrastructure of the vaginal epithelium of the ovariectomized mouse, was studied after oestradiol treatment. Tissue for electron microscopy was fixed at various times after injection in 2% osmium tetroxide and embedded in "Araldite". The earliest change seen in the vaginal epithelium after the intravaginal application of oestradiol occurred in nucleolar structure. In untreated animals nucleoli were small and of relatively uniform texture. After local application of oestradiol they increased in size and changed in appearance due to the development of large deeply staining intranucleolar bodies. The response became evident 1 hr after application and reached a maximum at 2-5 hr. Nucleoli from treated animals retained this structure up to the latest time of study (20 hr). Changes were also observed in mitochondrial number, cell and nuclear size, but these changes were small and inconsistent and occurred only at the later times. The significance of these results is discussed in relation to the action of oestradiol.

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