The elimination of O-linked glycans from glycoproteins under non-reducing conditions

Catherine A. Cooper; Nicolle H. Packer; John W. Redmond

doi:10.1007/BF00731156

The elimination of O-linked glycans from glycoproteins under non-reducing conditions

Catherine A. Cooper, Nicolle H. Packer, John W. Redmond^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

A simple procedure is described for the elimination of O-linked glycans from bovine submaxillary mucin under non-reducing conditions, using triethylamine in aqueous hydrazine. The glycans were isolated as the hydrazones, which were converted to the reducing glycans by exchange with acetone in neutral aqueous solution. The glycan alditols obtained after reduction corresponded to those obtained by the reductive β-elimination of O-glycans.

Original language	English
Pages (from-to)	163-167
Number of pages	5
Journal	Glycoconjugate Journal
Volume	11
Issue number	2
DOIs	https://doi.org/10.1007/BF00731156
Publication status	Published - Apr 1994

Keywords

β-elimination
glycoprotein
mucin
O-linked sugars
reducing sugars
sugar hydrazones

Access to Document

10.1007/BF00731156

Cite this

@article{4b038c4178564724a02782ffc9ff23da,

title = "The elimination of O-linked glycans from glycoproteins under non-reducing conditions",

abstract = "A simple procedure is described for the elimination of O-linked glycans from bovine submaxillary mucin under non-reducing conditions, using triethylamine in aqueous hydrazine. The glycans were isolated as the hydrazones, which were converted to the reducing glycans by exchange with acetone in neutral aqueous solution. The glycan alditols obtained after reduction corresponded to those obtained by the reductive β-elimination of O-glycans.",

keywords = "β-elimination, glycoprotein, mucin, O-linked sugars, reducing sugars, sugar hydrazones",

author = "Cooper, {Catherine A.} and Packer, {Nicolle H.} and Redmond, {John W.}",

year = "1994",

month = apr,

doi = "10.1007/BF00731156",

language = "English",

volume = "11",

pages = "163--167",

journal = "Glycoconjugate Journal",

issn = "0282-0080",

publisher = "Springer, Springer Nature",

number = "2",

}

TY - JOUR

T1 - The elimination of O-linked glycans from glycoproteins under non-reducing conditions

AU - Cooper, Catherine A.

AU - Packer, Nicolle H.

AU - Redmond, John W.

PY - 1994/4

Y1 - 1994/4

N2 - A simple procedure is described for the elimination of O-linked glycans from bovine submaxillary mucin under non-reducing conditions, using triethylamine in aqueous hydrazine. The glycans were isolated as the hydrazones, which were converted to the reducing glycans by exchange with acetone in neutral aqueous solution. The glycan alditols obtained after reduction corresponded to those obtained by the reductive β-elimination of O-glycans.

AB - A simple procedure is described for the elimination of O-linked glycans from bovine submaxillary mucin under non-reducing conditions, using triethylamine in aqueous hydrazine. The glycans were isolated as the hydrazones, which were converted to the reducing glycans by exchange with acetone in neutral aqueous solution. The glycan alditols obtained after reduction corresponded to those obtained by the reductive β-elimination of O-glycans.

KW - β-elimination

KW - glycoprotein

KW - mucin

KW - O-linked sugars

KW - reducing sugars

KW - sugar hydrazones

UR - http://www.scopus.com/inward/record.url?scp=0028111344&partnerID=8YFLogxK

U2 - 10.1007/BF00731156

DO - 10.1007/BF00731156

M3 - Article

C2 - 7804006

AN - SCOPUS:0028111344

SN - 0282-0080

VL - 11

SP - 163

EP - 167

JO - Glycoconjugate Journal

JF - Glycoconjugate Journal

IS - 2

ER -

The elimination of O-linked glycans from glycoproteins under non-reducing conditions

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this