The elimination of O-linked glycans from glycoproteins under non-reducing conditions

Catherine A. Cooper, Nicolle H. Packer, John W. Redmond*

*Corresponding author for this work

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A simple procedure is described for the elimination of O-linked glycans from bovine submaxillary mucin under non-reducing conditions, using triethylamine in aqueous hydrazine. The glycans were isolated as the hydrazones, which were converted to the reducing glycans by exchange with acetone in neutral aqueous solution. The glycan alditols obtained after reduction corresponded to those obtained by the reductive β-elimination of O-glycans.

Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalGlycoconjugate Journal
Volume11
Issue number2
DOIs
Publication statusPublished - Apr 1994

Keywords

  • β-elimination
  • glycoprotein
  • mucin
  • O-linked sugars
  • reducing sugars
  • sugar hydrazones

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