The fluidity of chloroplast thylakoid membranes and their constituent lipids. A comparative study by ESR

Roger G. Hiller*, John K. Raison

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    Comparative measurements were made of the fluidity of chloroplast thylakoids, total membrane lipids and polar lipids utilizing the order parameter and motion of spin labels. No significant differences were found in the fluidity of membranes or total membrane lipids from a wild type and a mutant barley (Hordeum vulgare chlorina f2 mutant) which lacks chlorophyll b and a 25 000 dalton thylakoid polypeptide. Redistribution of intrinsic, exoplasmic face (EF) membrane particles by unstacking thylakoid membranes in low salt medium also had no effect on membrane fluidity. However, heating of isolated thylakoids decreased membrane fluidity. The fluidity of vesicles composed of membrane lipids is much greater than that of the corresponding membranes. Fluidity of the membranes, however, increased during greening indicating that the rigidity of the membranes, compared with that of total membrane lipids, is not caused by chlorophyll or its associated peptides. It is concluded that the restriction of motion in the acyl chains in the thylakoids is not caused by chlorophyll or the major intrinsic polypeptide but by some other protein components.

    Original languageEnglish
    Pages (from-to)63-72
    Number of pages10
    JournalBBA - Biomembranes
    Volume599
    Issue number1
    DOIs
    Publication statusPublished - 20 Jun 1980

    Keywords

    • (Hordeum vulgare)
    • Chloroplast
    • Membrane lipid
    • Spin labeling
    • Thylakoid membrane

    Fingerprint

    Dive into the research topics of 'The fluidity of chloroplast thylakoid membranes and their constituent lipids. A comparative study by ESR'. Together they form a unique fingerprint.

    Cite this