The formin INF2 regulates basolateral-to-apical transcytosis and lumen formation in association with Cdc42 and MAL2

Ricardo Madrid*, Juan F. Aranda, Alejo E. Rodríguez-Fraticelli, Leandro Ventimiglia, Laura Andrés-Delgado, Mona Shehata, Susan Fanayan, Hamideh Shahheydari, Sergio Gómez, Alberto Jiménez, Fernando Martín-Belmonte, Jennifer A. Byrne, Miguel A. Alonso

*Corresponding author for this work

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Abstract

Transcytosis is a widespread pathway for apical targeting in epithelial cells. MAL2, an essential protein of the machinery for apical transcytosis, functions by shuttling in vesicular carriers between the apical zone and the cell periphery. We have identified INF2, an atypical formin with actin polymerization and depolymerization activities, which is a binding partner of MAL2. MAL2-positive vesicular carriers associate with short actin filaments during transcytosis in a process requiring INF2. INF2 binds Cdc42 in a GTP-loaded-dependent manner. Cdc42 and INF2 regulate MAL2 dynamics and are necessary for apical transcytosis and the formation of lateral lumens in hepatoma HepG2 cells. INF2 and MAL2 are also essential for the formation of the central lumen in organotypic cultures of epithelial MDCK cells. Our results reveal a functional mechanism whereby Cdc42, INF2, and MAL2 are sequentially ordered in a pathway dedicated to the regulation of transcytosis and lumen formation.

Original languageEnglish
Pages (from-to)814-827
Number of pages14
JournalDevelopmental Cell
Volume18
Issue number5
DOIs
Publication statusPublished - May 2010
Externally publishedYes

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Madrid, R., Aranda, J. F., Rodríguez-Fraticelli, A. E., Ventimiglia, L., Andrés-Delgado, L., Shehata, M., ... Alonso, M. A. (2010). The formin INF2 regulates basolateral-to-apical transcytosis and lumen formation in association with Cdc42 and MAL2. Developmental Cell, 18(5), 814-827. https://doi.org/10.1016/j.devcel.2010.04.001