The genome of Polaromonas sp strain JS666: insights into the evolution of a hydrocarbon- and xenobiotic-degrading bacterium, and features of relevance to biotechnology

Timothy E. Mattes*, Anne K. Alexander, Paul M. Richardson, A. Christine Munk, Cliff S. Han, Paul Stothard, Nicholas V. Coleman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

113 Citations (Scopus)

Abstract

Polaromonas sp. strain JS666 can grow on cis-1,2-dichloroethene (cDCE) as a sole carbon and energy source and may be useful for bioremediation of chlorinated solvent-contaminated sites. Analysis of the genome sequence of JS666 (5.9 Mb) shows a bacterium well adapted to pollution that carries many genes likely to be involved in hydrocarbon and xenobiotic catabolism and metal resistance. Clusters of genes coding for haloalkane, haloalkanoate, n-alkane, alicyclic acid, cyclic alcohol, and aromatic catabolism were analyzed in detail, and growth on acetate, catechol, chloroacetate, cyclohexane carboxylate, cyclohexanol, ferulate, heptane, 3-hydroxybenzoate, hydroxyquinol, gentisate, octane, protocatechuate, and salicylate was confirmed experimentally. Strain JS666 also harbors diverse putative mobile genetic elements, including retrons, inteins, a miniature inverted-repeat transposable element, insertion sequence transposases from 14 families, eight genomic islands, a Mu family bacteriophage, and two large (338- and 360-kb) plasmids. Both plasmids are likely to be self-transferable and carry genes for alkane, alcohol, aromatic, and haloacid metabolism. Overall, the JS666 genome sequence provides insights into the evolution of pollutant-degrading bacteria and provides a toolbox of catabolic genes with utility for biotechnology.

Original languageEnglish
Pages (from-to)6405-6416
Number of pages12
JournalApplied and Environmental Microbiology
Volume74
Issue number20
DOIs
Publication statusPublished - Oct 2008
Externally publishedYes

Keywords

  • XANTHOBACTER-AUTOTROPHICUS GJ10
  • BAEYER-VILLIGER MONOOXYGENASES
  • BETA-KETOADIPATE PATHWAY
  • MOBILE GENETIC ELEMENTS
  • PSEUDOMONAS-PUTIDA
  • CONTAMINATED GROUNDWATER
  • ORGANIC-COMPOUNDS
  • FERULIC ACID
  • DEGRADATION
  • BIODEGRADATION

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