The hand, foot and mouth disease virus capsid: sequence analysis and prediction of antigenic sites from homology modelling.

S. Ranganathan, S. Singh, C. L. Poh, V. T. Chow

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Enterovirus 71 (EV71) is the most common aetiological agent detected in cases of hand, foot and mouth disease (HFMD) resulting in incidences of neurological complications and fatality in recent years. A comparison of the capsid proteins implicated in the pathogenicity of the fatal and non-fatal strains of EV71, reveals a high degree of homology (93%-100% identity). To facilitate diagnostic immunoassays and vaccine development, a consensus structural model for the EV71 coat protein has been developed based primarily on the homologous structure of the bovine enterovirus. The overall architecture of the virion closely resembles those of related icosahedral picornaviruses. Detailed atomic modelling of the fatal 5865/SIN/00009 strain has been carried out, and the functional regions (known and predicted) from closely related viruses mapped onto the surface of the predicted structure. From the model, we have identified two putative immunogenic regions, one of which is unique to EV71. The hydrophobic pocket within VP1, found in bovine enterovirus, poliovirus and rhinovirus, is also conserved in EV71.

LanguageEnglish
Pages43-52
Number of pages10
JournalAppl Bioinformatics
Volume1
Issue number1
Publication statusPublished - 2002
Externally publishedYes

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hand, foot and mouth disease
Hand, Foot and Mouth Disease
Foot-and-Mouth Disease Virus
Enterovirus
capsid
Foot-and-mouth disease virus
Capsid
Viruses
Sequence Analysis
sequence analysis
Bovine Enterovirus
Proteins
Vaccines
prediction
Bovine enterovirus
Capsid Proteins
coat proteins
Picornaviridae
Rhinovirus
Poliovirus

Cite this

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title = "The hand, foot and mouth disease virus capsid: sequence analysis and prediction of antigenic sites from homology modelling.",
abstract = "Enterovirus 71 (EV71) is the most common aetiological agent detected in cases of hand, foot and mouth disease (HFMD) resulting in incidences of neurological complications and fatality in recent years. A comparison of the capsid proteins implicated in the pathogenicity of the fatal and non-fatal strains of EV71, reveals a high degree of homology (93{\%}-100{\%} identity). To facilitate diagnostic immunoassays and vaccine development, a consensus structural model for the EV71 coat protein has been developed based primarily on the homologous structure of the bovine enterovirus. The overall architecture of the virion closely resembles those of related icosahedral picornaviruses. Detailed atomic modelling of the fatal 5865/SIN/00009 strain has been carried out, and the functional regions (known and predicted) from closely related viruses mapped onto the surface of the predicted structure. From the model, we have identified two putative immunogenic regions, one of which is unique to EV71. The hydrophobic pocket within VP1, found in bovine enterovirus, poliovirus and rhinovirus, is also conserved in EV71.",
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The hand, foot and mouth disease virus capsid : sequence analysis and prediction of antigenic sites from homology modelling. / Ranganathan, S.; Singh, S.; Poh, C. L.; Chow, V. T.

In: Appl Bioinformatics, Vol. 1, No. 1, 2002, p. 43-52.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - The hand, foot and mouth disease virus capsid

T2 - Appl Bioinformatics

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AU - Singh, S.

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AU - Chow, V. T.

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