The highly O-glycosylated glycoprotein gp2 of equine herpesvirus 1 is encoded by gene 71

Janet E. Wellington; George P. Allen; Andrew A. Gooley; Daria N. Love; Nicolle H. Packer; Jun X. Yan; J. Millar Whalley

The highly O-glycosylated glycoprotein gp2 of equine herpesvirus 1 is encoded by gene 71

Janet E. Wellington, George P. Allen, Andrew A. Gooley, Daria N. Love, Nicolle H. Packer, Jun X. Yan, J. Millar Whalley^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

There have been conflicting reports regarding the gene assignment of the high-molecular-mass envelope glycoprotein gp2 (gp300) of equine herpesvirus 1. Here, we provide an unequivocal demonstration that gp2 is encoded by gene 71. gp2 that was purified with a defining monoclonal antibody was cleaved internally to yield a 42-kDa protein encoded by gene 71. Amino acid composition data and N-terminal sequence analysis of a tryptic peptide identified gp2 as the product of equine herpesvirus 1 gene 71 with the SWISS- PROT database. Analysis of gp2's monosaccharide composition and the 42-kDa subunit showed that the high level of O glycosylation occurs on the serine/threonine-rich region upstream of the cleavage site.

Original language	English
Pages (from-to)	8195-8198
Number of pages	4
Journal	Journal of Virology
Volume	70
Issue number	11
Publication status	Published - Nov 1996

Cite this

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title = "The highly O-glycosylated glycoprotein gp2 of equine herpesvirus 1 is encoded by gene 71",

abstract = "There have been conflicting reports regarding the gene assignment of the high-molecular-mass envelope glycoprotein gp2 (gp300) of equine herpesvirus 1. Here, we provide an unequivocal demonstration that gp2 is encoded by gene 71. gp2 that was purified with a defining monoclonal antibody was cleaved internally to yield a 42-kDa protein encoded by gene 71. Amino acid composition data and N-terminal sequence analysis of a tryptic peptide identified gp2 as the product of equine herpesvirus 1 gene 71 with the SWISS- PROT database. Analysis of gp2's monosaccharide composition and the 42-kDa subunit showed that the high level of O glycosylation occurs on the serine/threonine-rich region upstream of the cleavage site.",

author = "Wellington, {Janet E.} and Allen, {George P.} and Gooley, {Andrew A.} and Love, {Daria N.} and Packer, {Nicolle H.} and Yan, {Jun X.} and Whalley, {J. Millar}",

year = "1996",

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journal = "Journal of Virology",

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T1 - The highly O-glycosylated glycoprotein gp2 of equine herpesvirus 1 is encoded by gene 71

AU - Wellington, Janet E.

AU - Allen, George P.

AU - Gooley, Andrew A.

AU - Love, Daria N.

AU - Packer, Nicolle H.

AU - Yan, Jun X.

AU - Whalley, J. Millar

PY - 1996/11

Y1 - 1996/11

N2 - There have been conflicting reports regarding the gene assignment of the high-molecular-mass envelope glycoprotein gp2 (gp300) of equine herpesvirus 1. Here, we provide an unequivocal demonstration that gp2 is encoded by gene 71. gp2 that was purified with a defining monoclonal antibody was cleaved internally to yield a 42-kDa protein encoded by gene 71. Amino acid composition data and N-terminal sequence analysis of a tryptic peptide identified gp2 as the product of equine herpesvirus 1 gene 71 with the SWISS- PROT database. Analysis of gp2's monosaccharide composition and the 42-kDa subunit showed that the high level of O glycosylation occurs on the serine/threonine-rich region upstream of the cleavage site.

AB - There have been conflicting reports regarding the gene assignment of the high-molecular-mass envelope glycoprotein gp2 (gp300) of equine herpesvirus 1. Here, we provide an unequivocal demonstration that gp2 is encoded by gene 71. gp2 that was purified with a defining monoclonal antibody was cleaved internally to yield a 42-kDa protein encoded by gene 71. Amino acid composition data and N-terminal sequence analysis of a tryptic peptide identified gp2 as the product of equine herpesvirus 1 gene 71 with the SWISS- PROT database. Analysis of gp2's monosaccharide composition and the 42-kDa subunit showed that the high level of O glycosylation occurs on the serine/threonine-rich region upstream of the cleavage site.

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AN - SCOPUS:0029845806

SN - 0022-538X

VL - 70

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JO - Journal of Virology

JF - Journal of Virology

IS - 11

ER -

The highly O-glycosylated glycoprotein gp2 of equine herpesvirus 1 is encoded by gene 71

Abstract

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