The highly O-glycosylated glycoprotein gp2 of equine herpesvirus 1 is encoded by gene 71

Janet E. Wellington, George P. Allen, Andrew A. Gooley, Daria N. Love, Nicolle H. Packer, Jun X. Yan, J. Millar Whalley*

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

There have been conflicting reports regarding the gene assignment of the high-molecular-mass envelope glycoprotein gp2 (gp300) of equine herpesvirus 1. Here, we provide an unequivocal demonstration that gp2 is encoded by gene 71. gp2 that was purified with a defining monoclonal antibody was cleaved internally to yield a 42-kDa protein encoded by gene 71. Amino acid composition data and N-terminal sequence analysis of a tryptic peptide identified gp2 as the product of equine herpesvirus 1 gene 71 with the SWISS- PROT database. Analysis of gp2's monosaccharide composition and the 42-kDa subunit showed that the high level of O glycosylation occurs on the serine/threonine-rich region upstream of the cleavage site.

Original languageEnglish
Pages (from-to)8195-8198
Number of pages4
JournalJournal of Virology
Volume70
Issue number11
Publication statusPublished - Nov 1996

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