TY - JOUR
T1 - The impact of Zika virus exposure on the placental proteomic profile
AU - Rosa-Fernandes, Livia
AU - Bandeira, Carla
AU - Pour, Shahab Zaki
AU - Benedetti, Viviane de Fátima
AU - Ferreira, Daniel
AU - Lorenzon, Aline R.
AU - Moreli, Jusciele B.
AU - Marinho, Claudio Romero Farias
AU - Larsen, Martin R.
AU - Zanotto, Paolo
AU - Palmisano, Giuseppe
AU - Bevilacqua, Estela
PY - 2022/1/1
Y1 - 2022/1/1
N2 - Zika virus (ZIKV) infection has caused severe unexpected clinical outcomes in neonates and adults during the recent outbreak in Latin America, particularly in Brazil. Congenital malformations associated with ZIKV have been frequently reported; nevertheless, the mechanism of vertical transmission and the involvement of placental cells remains unclear. In this study, we applied quantitative proteomics analysis in a floating explant model of chorionic villi of human placental tissues incubated with ZIKV and with ZIKV pre-adsorbed with anti-ZIKV envelope protein. Proteomic data are available via ProteomeXchange with identifier PXD025764. Altered levels of proteins were involved in cell proliferation, apoptosis, inflammatory processes, and the integrin-cytoskeleton complex. Antibody-opsonized ZIKV particles differentially modulated the pattern of protein expression in placental cells; this phenomenon may play a pivotal role in determining the course of infection and the role of mixed infections. The expression of specific proteins was also evaluated by immunoperoxidase assays. These data fill gaps in our understanding of early events after ZIKV placental exposure and help identify infection control targets.
AB - Zika virus (ZIKV) infection has caused severe unexpected clinical outcomes in neonates and adults during the recent outbreak in Latin America, particularly in Brazil. Congenital malformations associated with ZIKV have been frequently reported; nevertheless, the mechanism of vertical transmission and the involvement of placental cells remains unclear. In this study, we applied quantitative proteomics analysis in a floating explant model of chorionic villi of human placental tissues incubated with ZIKV and with ZIKV pre-adsorbed with anti-ZIKV envelope protein. Proteomic data are available via ProteomeXchange with identifier PXD025764. Altered levels of proteins were involved in cell proliferation, apoptosis, inflammatory processes, and the integrin-cytoskeleton complex. Antibody-opsonized ZIKV particles differentially modulated the pattern of protein expression in placental cells; this phenomenon may play a pivotal role in determining the course of infection and the role of mixed infections. The expression of specific proteins was also evaluated by immunoperoxidase assays. These data fill gaps in our understanding of early events after ZIKV placental exposure and help identify infection control targets.
KW - Antibody-mediated infection
KW - Cell death
KW - Chorionic villi
KW - Placenta
KW - Proteomics
KW - Zika virus
UR - http://www.scopus.com/inward/record.url?scp=85116214269&partnerID=8YFLogxK
U2 - 10.1016/j.bbadis.2021.166270
DO - 10.1016/j.bbadis.2021.166270
M3 - Article
C2 - 34582966
AN - SCOPUS:85116214269
SN - 0925-4439
VL - 1868
SP - 1
EP - 12
JO - Biochimica et Biophysica Acta - Molecular Basis of Disease
JF - Biochimica et Biophysica Acta - Molecular Basis of Disease
IS - 1
M1 - 166270
ER -