The influence hydroxyapatite nanoparticle shape and size on the properties of biphasic calcium phosphate scaffolds coated with hydroxyapatite-PCL composites

Seyed Iman Roohani-Esfahani, Saied Nouri-Khorasani, Zufu Lu, Richard Appleyard, Hala Zreiqat*

*Corresponding author for this work

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

We developed a composite biphasic calcium phosphate (BCP) scaffold by coating a nanocomposite layer, consisting of hydroxyapatite (HA) nanoparticles and polycaprolactone (PCL), over the surface of BCP. The effects of HA particle size and shape in the coating layer on the mechanical and biological properties of the BCP scaffold were examined. Micro-computerized tomography studies showed that the prepared scaffolds were highly porous (∼91%) with large pore size (400-700 μm) and an interconnected porous network of ∼100%. The HA nanoparticle (needle shape)-composite coated scaffolds displayed the highest compressive strength (2.1 ± 0.17 MPa), compared to pure HA/β-TCP (0.1 ± 0.05 MPa) and to the micron HA - composite coated scaffolds (0.29 ± 0.07 MPa). These needle shaped scaffolds also showed enhanced elasticity and similar stress-strain profile to natural bone. Needle shaped coated HA/PCL particles induced the differentiation of primary human bone derived cells, with significant upregulation of osteogenic gene expression (Runx2, collagen type I, osteocalcin and bone sialoprotein) and alkaline phosphatase activity compared to other groups. These properties are essential for enhancing bone ingrowth in load-bearing applications. The developed composite scaffolds possessed superior physical, mechanical, elastic and biological properties rendering them potentially useful for bone tissue regeneration.

Original languageEnglish
Pages (from-to)5498-5509
Number of pages12
JournalBiomaterials
Volume31
Issue number21
DOIs
Publication statusPublished - Jul 2010
Externally publishedYes

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