The influence of different types of upconversion nanoparticles surface coatings on neurotoxicity

E. V. Mitroshina; T. A. Mishchenko; M. V. Vedunova; A. V. Yudintsev; A. N. Generalova; A. V. Nechaev; S. M. Deyev; I. V. Mukhina; Andrei V. Zvyagin

doi:10.17691/stm2016.8.4.18

The influence of different types of upconversion nanoparticles surface coatings on neurotoxicity

E. V. Mitroshina, T. A. Mishchenko, M. V. Vedunova, A. V. Yudintsev, A. N. Generalova, A. V. Nechaev, S. M. Deyev, I. V. Mukhina, Andrei V. Zvyagin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The aim of the investigation was to study the effect of upconversion nanoparticles (UCNPs) with different surface coatings on viability and functional neural network activity of primary hippocampal cultures. Materials and Methods. The UCNPs with three surface coating modifications (tetramethylammonium hydroxide (TMAH), polymaleic acid octadecene and polyethyleneimine (PEI)) were synthesized by a solvothermal technique with further hydrophilization. Primary hippocampal cultures, obtained from C57BL/6 mice embryos (E18), were incubated with tested UCNPs in the concentration of 0.8 and 8 mg/ml during 72 h. The cell viability detection, evaluation of morphological changes by immunocytochemical staining as well as the UCNPs influence on the main parameters of the functional neural network calcium activity and on the endocytosis processes were carried out. Results. Our studies revealed that UCNPs caused a dose-dependent cytotoxic effect on primary hippocampal cultures, wherein the severity of this effect directly related to the type of UCNPs surface coating. The greatest cytotoxicity was identified for UCNP-PEI, and the least — for UCNP-TMAH. UCNPs toxicity is manifested in significant morphological changes of neural networks and in the increase the number of dead cells (p<0.05) in primary hippocampal cultures. Moreover, a significant decrease (p<0.05) in the main parameters of spontaneous functional calcium activity was shown. Conclusion. A comprehensive investigation of the nanoparticles effects on primary hippocampal cultures showed that all tested UCNPs have the strong toxic effect to the nervous system cells.

Original language	English
Pages (from-to)	133-141
Number of pages	9
Journal	Sovremennye Tehnologii v Medicine
Volume	8
Issue number	4
DOIs	https://doi.org/10.17691/stm2016.8.4.18
Publication status	Published - 2016

Keywords

Functional network activity
Neural network
Particle toxicology
Primary hippocampal cultures
Upconversion nanoparticles

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10.17691/stm2016.8.4.18

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title = "The influence of different types of upconversion nanoparticles surface coatings on neurotoxicity",

abstract = "The aim of the investigation was to study the effect of upconversion nanoparticles (UCNPs) with different surface coatings on viability and functional neural network activity of primary hippocampal cultures. Materials and Methods. The UCNPs with three surface coating modifications (tetramethylammonium hydroxide (TMAH), polymaleic acid octadecene and polyethyleneimine (PEI)) were synthesized by a solvothermal technique with further hydrophilization. Primary hippocampal cultures, obtained from C57BL/6 mice embryos (E18), were incubated with tested UCNPs in the concentration of 0.8 and 8 mg/ml during 72 h. The cell viability detection, evaluation of morphological changes by immunocytochemical staining as well as the UCNPs influence on the main parameters of the functional neural network calcium activity and on the endocytosis processes were carried out. Results. Our studies revealed that UCNPs caused a dose-dependent cytotoxic effect on primary hippocampal cultures, wherein the severity of this effect directly related to the type of UCNPs surface coating. The greatest cytotoxicity was identified for UCNP-PEI, and the least — for UCNP-TMAH. UCNPs toxicity is manifested in significant morphological changes of neural networks and in the increase the number of dead cells (p<0.05) in primary hippocampal cultures. Moreover, a significant decrease (p<0.05) in the main parameters of spontaneous functional calcium activity was shown. Conclusion. A comprehensive investigation of the nanoparticles effects on primary hippocampal cultures showed that all tested UCNPs have the strong toxic effect to the nervous system cells.",

keywords = "Functional network activity, Neural network, Particle toxicology, Primary hippocampal cultures, Upconversion nanoparticles",

author = "Mitroshina, {E. V.} and Mishchenko, {T. A.} and Vedunova, {M. V.} and Yudintsev, {A. V.} and Generalova, {A. N.} and Nechaev, {A. V.} and Deyev, {S. M.} and Mukhina, {I. V.} and Zvyagin, {Andrei V.}",

year = "2016",

doi = "10.17691/stm2016.8.4.18",

language = "English",

volume = "8",

pages = "133--141",

journal = "Sovremennye Tehnologii v Medicine",

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publisher = "Nizhny Novgorod State Medical Academy of the Ministry of Health of the Russian Federation",

number = "4",

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T1 - The influence of different types of upconversion nanoparticles surface coatings on neurotoxicity

AU - Mitroshina, E. V.

AU - Mishchenko, T. A.

AU - Vedunova, M. V.

AU - Yudintsev, A. V.

AU - Generalova, A. N.

AU - Nechaev, A. V.

AU - Deyev, S. M.

AU - Mukhina, I. V.

AU - Zvyagin, Andrei V.

PY - 2016

Y1 - 2016

N2 - The aim of the investigation was to study the effect of upconversion nanoparticles (UCNPs) with different surface coatings on viability and functional neural network activity of primary hippocampal cultures. Materials and Methods. The UCNPs with three surface coating modifications (tetramethylammonium hydroxide (TMAH), polymaleic acid octadecene and polyethyleneimine (PEI)) were synthesized by a solvothermal technique with further hydrophilization. Primary hippocampal cultures, obtained from C57BL/6 mice embryos (E18), were incubated with tested UCNPs in the concentration of 0.8 and 8 mg/ml during 72 h. The cell viability detection, evaluation of morphological changes by immunocytochemical staining as well as the UCNPs influence on the main parameters of the functional neural network calcium activity and on the endocytosis processes were carried out. Results. Our studies revealed that UCNPs caused a dose-dependent cytotoxic effect on primary hippocampal cultures, wherein the severity of this effect directly related to the type of UCNPs surface coating. The greatest cytotoxicity was identified for UCNP-PEI, and the least — for UCNP-TMAH. UCNPs toxicity is manifested in significant morphological changes of neural networks and in the increase the number of dead cells (p<0.05) in primary hippocampal cultures. Moreover, a significant decrease (p<0.05) in the main parameters of spontaneous functional calcium activity was shown. Conclusion. A comprehensive investigation of the nanoparticles effects on primary hippocampal cultures showed that all tested UCNPs have the strong toxic effect to the nervous system cells.

AB - The aim of the investigation was to study the effect of upconversion nanoparticles (UCNPs) with different surface coatings on viability and functional neural network activity of primary hippocampal cultures. Materials and Methods. The UCNPs with three surface coating modifications (tetramethylammonium hydroxide (TMAH), polymaleic acid octadecene and polyethyleneimine (PEI)) were synthesized by a solvothermal technique with further hydrophilization. Primary hippocampal cultures, obtained from C57BL/6 mice embryos (E18), were incubated with tested UCNPs in the concentration of 0.8 and 8 mg/ml during 72 h. The cell viability detection, evaluation of morphological changes by immunocytochemical staining as well as the UCNPs influence on the main parameters of the functional neural network calcium activity and on the endocytosis processes were carried out. Results. Our studies revealed that UCNPs caused a dose-dependent cytotoxic effect on primary hippocampal cultures, wherein the severity of this effect directly related to the type of UCNPs surface coating. The greatest cytotoxicity was identified for UCNP-PEI, and the least — for UCNP-TMAH. UCNPs toxicity is manifested in significant morphological changes of neural networks and in the increase the number of dead cells (p<0.05) in primary hippocampal cultures. Moreover, a significant decrease (p<0.05) in the main parameters of spontaneous functional calcium activity was shown. Conclusion. A comprehensive investigation of the nanoparticles effects on primary hippocampal cultures showed that all tested UCNPs have the strong toxic effect to the nervous system cells.

KW - Functional network activity

KW - Neural network

KW - Particle toxicology

KW - Primary hippocampal cultures

KW - Upconversion nanoparticles

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DO - 10.17691/stm2016.8.4.18

M3 - Article

AN - SCOPUS:85007439946

SN - 2076-4243

VL - 8

SP - 133

EP - 141

JO - Sovremennye Tehnologii v Medicine

JF - Sovremennye Tehnologii v Medicine

IS - 4

ER -

The influence of different types of upconversion nanoparticles surface coatings on neurotoxicity

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Keywords

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