The minimum information required for a glycomics experiment (MIRAGE) project: sample preparation guidelines for reliable reporting of glycomics datasets

Weston B. Struwe*, Sanjay Agravat, Kiyoko F. Aoki-Kinoshita, Matthew P. Campbell, Catherine E. Costello, Anne Dell, Ten Feizi, Stuart M. Haslam, Niclas G. Karlsson, Kay Hooi Khoo, Daniel Kolarich, Yan Liu, Ryan Mcbride, Milos V. Novotny, Nicolle H. Packer, James C. Paulson, Erdmann Rapp, Rene Ranzinger, Pauline M. Rudd, David F. SmithMichael Tiemeyer, Lance Wells, William S. York, Joseph Zaia, Carsten Kettner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

63 Citations (Scopus)
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Abstract

The minimum information required for a glycomics experiment (MIRAGE) project was established in 2011 to provide guidelines to aid in data reporting from all types of experiments in glycomics research including mass spectrometry (MS), liquid chromatography, glycan arrays, data handling and sample preparation. MIRAGE is a concerted effort of the wider glycomics community that considers the adaptation of reporting guidelines as an important step towards critical evaluation and dissemination of datasets as well as broadening of experimental techniques worldwide. The MIRAGE Commission published reporting guidelines for MS data and here we outline guidelines for sample preparation. The sample preparation guidelines include all aspects of sample generation, purification and modification from biological and/or synthetic carbohydrate material. The application of MIRAGE sample preparation guidelines will lead to improved recording of experimental protocols and reporting of understandable and reproducible glycomics datasets.

Original languageEnglish
Pages (from-to)907-910
Number of pages4
JournalGlycobiology
Volume26
Issue number9
DOIs
Publication statusPublished - 1 Sept 2016

Bibliographical note

Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • glycobiology
  • glycomics
  • MIRAGE
  • sample preparation

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