The mitochondrial genome of the soybean cyst nematode, Heterodera glycines

Tracey Gibson, Daniel Farrugia, Jeff Barrett, David J. Chitwood, Janet Rowe, Sergei Subbotin, Mark Dowton*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

We sequenced the entire coding region of the mitochondrial genome of Heterodera glycines. The sequence obtained comprised 14.9kb, with PCR evidence indicating that the entire genome comprised a single, circular molecule of approximately 21-22kb. The genome is the most T-rich nematode mitochondrial genome reported to date, with T representing over half of all nucleotides on the coding strand. The genome also contains the highest number of poly(T) tracts so far reported (to our knowledge), with 60 poly(T) tracts ≥ 12 Ts. All genes are transcribed from the same mitochondrial strand. The organization of the mitochondrial genome of H. glycines shows a number of similarities compared with Radopholus similis, but fewer similarities when compared with Meloidogyne javanica. Very few gene boundaries are shared with Globodera pallida or Globodera rostochiensis. Partial mitochondrial genome sequences were also obtained for Heterodera cardiolata (5.3kb) and Punctodera chalcoensis (6.8kb), and these had identical organizations compared with H. glycines. We found PCR evidence of a minicircular mitochondrial genome in P. chalcoensis, but at low levels and lacking a noncoding region. Such circularised genome fragments may be present at low levels in a range of nematodes, with multipartite mitochondrial genomes representing a shift to a condition in which these subgenomic circles predominate.

Original languageEnglish
Pages (from-to)565-574
Number of pages10
JournalGenome
Volume54
Issue number7
DOIs
Publication statusPublished - Jul 2011

Keywords

  • gene rearrangement
  • Heterodera cardiolata
  • Heteroderidae
  • mitochondrial
  • nematode
  • Punctodera
  • Punctoderinae

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