The mRNA-based reprogramming of fibroblasts from a SOD1E101G familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007

Rachelle Balez; Tracey Berg; Monique Bax; Sonia Sanz Muñoz; Mauricio C. Cabral-da-Silva; Martin Engel; Dzung Do-Ha; Claire H. Stevens; Dominic Rowe; Shu Yang; Ian P. Blair; Lezanne Ooi

doi:10.1016/j.scr.2020.101701

The mRNA-based reprogramming of fibroblasts from a SOD1^E101G familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007

Rachelle Balez, Tracey Berg, Monique Bax, Sonia Sanz Muñoz, Mauricio C. Cabral-da-Silva, Martin Engel, Dzung Do-Ha, Claire H. Stevens, Dominic Rowe, Shu Yang, Ian P. Blair, Lezanne Ooi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

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Abstract

Dermal fibroblasts were donated by a 43 year old male patient with clinically diagnosed familial amyotrophic lateral sclerosis (ALS), carrying the SOD1^E101G mutation. The induced pluripotent stem cell (iPSC) line UOWi007-A was generated using repeated mRNA transfections for pluripotency transcription factors Oct4, Klf4, Sox2, c-Myc, Lin28 and Nanog. The iPSCs carried the SOD1^E101G genotype and had a normal karyotype, expressed expected pluripotency markers and were capable of in vitro differentiation into endodermal, mesodermal and ectodermal lineages. This iPSC line may be useful for investigating familial ALS resulting from a SOD1 ^E101G mutation.

Original language	English
Article number	101701
Pages (from-to)	1-4
Number of pages	4
Journal	Stem Cell Research
Volume	42
DOIs	https://doi.org/10.1016/j.scr.2020.101701
Publication status	Published - 1 Jan 2020

Bibliographical note

Copyright the Author(s) 2020. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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10.1016/j.scr.2020.101701

Publisher version (open access)Final published version, 1.31 MBLicence: CC BY-NC-ND

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Balez, R., Berg, T., Bax, M., Muñoz, S. S., Cabral-da-Silva, M. C., Engel, M., Do-Ha, D., Stevens, C. H., Rowe, D., Yang, S., Blair, I. P., & Ooi, L. (2020). The mRNA-based reprogramming of fibroblasts from a SOD1^E101G familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007. Stem Cell Research, 42, 1-4. Article 101701. https://doi.org/10.1016/j.scr.2020.101701

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title = "The mRNA-based reprogramming of fibroblasts from a SOD1E101G familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007",

abstract = "Dermal fibroblasts were donated by a 43 year old male patient with clinically diagnosed familial amyotrophic lateral sclerosis (ALS), carrying the SOD1E101G mutation. The induced pluripotent stem cell (iPSC) line UOWi007-A was generated using repeated mRNA transfections for pluripotency transcription factors Oct4, Klf4, Sox2, c-Myc, Lin28 and Nanog. The iPSCs carried the SOD1E101G genotype and had a normal karyotype, expressed expected pluripotency markers and were capable of in vitro differentiation into endodermal, mesodermal and ectodermal lineages. This iPSC line may be useful for investigating familial ALS resulting from a SOD1 E101G mutation.",

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year = "2020",

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doi = "10.1016/j.scr.2020.101701",

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Balez, R, Berg, T, Bax, M, Muñoz, SS, Cabral-da-Silva, MC, Engel, M, Do-Ha, D, Stevens, CH, Rowe, D , Yang, S , Blair, IP & Ooi, L 2020, 'The mRNA-based reprogramming of fibroblasts from a SOD1^E101G familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007', Stem Cell Research, vol. 42, 101701, pp. 1-4. https://doi.org/10.1016/j.scr.2020.101701

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AU - Balez, Rachelle

AU - Berg, Tracey

AU - Bax, Monique

AU - Muñoz, Sonia Sanz

AU - Cabral-da-Silva, Mauricio C.

AU - Engel, Martin

AU - Do-Ha, Dzung

AU - Stevens, Claire H.

AU - Rowe, Dominic

AU - Yang, Shu

AU - Blair, Ian P.

AU - Ooi, Lezanne

N1 - Copyright the Author(s) 2020. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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Y1 - 2020/1/1

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AB - Dermal fibroblasts were donated by a 43 year old male patient with clinically diagnosed familial amyotrophic lateral sclerosis (ALS), carrying the SOD1E101G mutation. The induced pluripotent stem cell (iPSC) line UOWi007-A was generated using repeated mRNA transfections for pluripotency transcription factors Oct4, Klf4, Sox2, c-Myc, Lin28 and Nanog. The iPSCs carried the SOD1E101G genotype and had a normal karyotype, expressed expected pluripotency markers and were capable of in vitro differentiation into endodermal, mesodermal and ectodermal lineages. This iPSC line may be useful for investigating familial ALS resulting from a SOD1 E101G mutation.

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The mRNA-based reprogramming of fibroblasts from a SOD1^E101G familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007

Abstract

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Developing insight into the molecular origins of familial and sporadic frontotemporal dementia and amyotrophic lateral sclerosis

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The mRNA-based reprogramming of fibroblasts from a SOD1E101G familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007

Abstract

Bibliographical note

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Developing insight into the molecular origins of familial and sporadic frontotemporal dementia and amyotrophic lateral sclerosis

Cite this

The mRNA-based reprogramming of fibroblasts from a SOD1^E101G familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007