The orexin-1 receptor antagonist SB-334867 attenuates anxiety in rats exposed to cat odor but not the elevated plus maze

An investigation of Trial 1 and Trial 2 effects

Lauren G. Staples*, Jennifer L. Cornish

*Corresponding author for this work

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The orexins are hypothalamic neuropeptides most well known for their roles in regulating feeding and sleeping behaviors. Recent findings suggest that orexin-A may also modulate anxiety, although how and when the orexin system is involved remains unclear. To address this, we investigated the dose-dependent effects of the orexin-1 receptor antagonist SB-334867 in two rodent models of anxiety: the cat odor avoidance model and the elevated plus maze. In both models we tested the effects of SB-334867 when anxiety is novel (Trial 1) and familiar (Trial 2). In the first experiment, Wistar rats were treated with vehicle or SB-334867 (5, 10 or 20. mg/kg, i.p.) prior to their first or second exposure to cat odor. During Trial 1, rats treated with 10. mg/kg of SB-334867 approached the cat odor stimulus more than vehicle-treated rats. During Trial 2 the effects were more marked, with 10. mg/kg of SB-334867 increasing approach times, increasing the number of times rats exited the hide box to engage in exploratory behavior, and decreasing overall hide times. In addition, the 20. mg/kg dose decreased general activity during Trial 2. In the second experiment, the effects of SB-334867 (10 and 20. mg/kg) were tested in the elevated plus maze. There were no significant differences produced by drug treatment during either Trial 1 or Trial 2. Results suggest that SB-334867 decreases anxiety induced by some, but not all, stressors.

Original languageEnglish
Pages (from-to)294-300
Number of pages7
JournalHormones and Behavior
Volume65
Issue number3
DOIs
Publication statusPublished - Mar 2014

Keywords

  • Anxiety
  • Elevated plus maze
  • Hypocretins
  • One trial tolerance
  • Orexins
  • Predator odor avoidance
  • SB-334867

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