The proteomes of Sydney rock oysters vary spatially according to exposure to acid sulfate runoff

Valter Amaral, Emma L. Thompson, Melanie J. Bishop, David A. Raftos

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Runoff from acid sulfate soils (ASS) has severe environmental and economic impacts on estuarine ecosystems. Oysters display reduced abundance, growth rate and shell thickness when exposed to ASS runoff, yet the molecular underpinnings of their responses have not been explored. We hypothesised that the proteomes of wild Sydney rock oysters, Saccostrea glomerata, would differ between populations recurrently exposed to ASS compared with those unaffected by runoff from ASS. We used two-dimensional electrophoresis to compare protein abundances in the gills of S. glomerata collected from two sites close to (acidified) and two sites away from (reference) major ASS outflow drains in a south-east Australian estuary. Approximately 5% of the proteome was differentially expressed between oysters from acidified and reference sites, with five protein spots more abundant and one less abundant at the sites close to drains. Another protein spot was present only in oysters from reference sites. This study is the first screening of spatial variation in the protein expression of S. glomerata with respect to discharge from ASS. Altered protein expression may underpin short-term inducible responses to ASS runoff, or genetic resistance acquired through recurrent exposure of populations to the stressor.

LanguageEnglish
Pages361-369
Number of pages9
JournalMarine and Freshwater Research
Volume63
Issue number4
DOIs
Publication statusPublished - 2012

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Saccostrea glomerata
acid sulfate soils
acid sulfate soil
proteome
sulfates
runoff
sulfate
acids
acid
rock
protein
oysters
at-risk population
drain
protein synthesis
estuarine ecosystem
shell (molluscs)
proteins
genetic resistance
economic impact

Cite this

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title = "The proteomes of Sydney rock oysters vary spatially according to exposure to acid sulfate runoff",
abstract = "Runoff from acid sulfate soils (ASS) has severe environmental and economic impacts on estuarine ecosystems. Oysters display reduced abundance, growth rate and shell thickness when exposed to ASS runoff, yet the molecular underpinnings of their responses have not been explored. We hypothesised that the proteomes of wild Sydney rock oysters, Saccostrea glomerata, would differ between populations recurrently exposed to ASS compared with those unaffected by runoff from ASS. We used two-dimensional electrophoresis to compare protein abundances in the gills of S. glomerata collected from two sites close to (acidified) and two sites away from (reference) major ASS outflow drains in a south-east Australian estuary. Approximately 5{\%} of the proteome was differentially expressed between oysters from acidified and reference sites, with five protein spots more abundant and one less abundant at the sites close to drains. Another protein spot was present only in oysters from reference sites. This study is the first screening of spatial variation in the protein expression of S. glomerata with respect to discharge from ASS. Altered protein expression may underpin short-term inducible responses to ASS runoff, or genetic resistance acquired through recurrent exposure of populations to the stressor.",
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The proteomes of Sydney rock oysters vary spatially according to exposure to acid sulfate runoff. / Amaral, Valter; Thompson, Emma L.; Bishop, Melanie J.; Raftos, David A.

In: Marine and Freshwater Research, Vol. 63, No. 4, 2012, p. 361-369.

Research output: Contribution to journalArticleResearchpeer-review

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