The role of zinc efflux during Acinetobacter baumannii infection

Saleh F. Alquethamy, Felise G. Adams, Varsha Naidu, Marjan Khorvash, Victoria G. Pederick, Maoge Zang, James C. Paton, Ian T. Paulsen, Karl A. Hassan, Amy K. Cain, Christopher A. McDevitt, Bart A. Eijkelkamp

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    Acinetobacter baumannii is a ubiquitous Gram-negative bacterium, that is associated with significant disease in immunocompromised individuals. The success of A. baumannii is partly attributable to its high level of antibiotic resistance. Further, A. baumannii expresses a broad arsenal of putative zinc efflux systems that are likely to aid environmental persistence and host colonization, but detailed insights into how the bacterium deals with toxic concentrations of zinc are lacking. In this study we present the transcriptomic responses of A. baumannii to toxic zinc concentrations. Subsequent mutant analyses revealed a primary role for the resistance-nodulation-cell division heavy metal efflux system CzcCBA, and the cation diffusion facilitator transporter CzcD in zinc resistance. To examine the role of zinc at the host-pathogen interface we utilized a murine model of zinc deficiency and challenge with wild-type and czcA mutant strains, which identified highly site-specific roles for zinc during A. baumannii infection. Overall, we provide novel insight into the key zinc resistance mechanisms of A. baumannii and outline the role these systems play in enabling the bacterium to survive in diverse environments.

    Original languageEnglish
    Pages (from-to)150-158
    Number of pages9
    JournalACS Infectious Diseases
    Volume6
    Issue number1
    Early online date28 Oct 2019
    DOIs
    Publication statusPublished - 10 Jan 2020

    Keywords

    • metal ions
    • nutrition
    • infection
    • transporters
    • pneumonia

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