The secondary structure content of pigment-protein complexes from the thylakoids of two Chromophyte algae

Roger G. Hiller*, Anne Marie Bardin, Eliane Nabedryk

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The light-harvesting complex and Photosystem I have been isolated from thylakoids of two chromophyte algae by digitonin solubilisation and sucrose-gradient centrifugation. The α-helic and β-structure content was determined by ultraviolet circular dichroism. The values obtained were approx. 40% α-helix and approx. 14% β-structure for the light-harvesting complex of both Pavlova lutherii and Phaeodactylum tricornutum. For Photosystem I the values were approx. 55% α-helix and 7% β-structure for both algae. It is concluded that for all photosynthetic antennae containing chlorophyll the dominant secondary structure is α-helix.

Original languageEnglish
Pages (from-to)365-369
Number of pages5
JournalBBA - Bioenergetics
Volume894
Issue number3
DOIs
Publication statusPublished - 17 Dec 1987
Externally publishedYes

Keywords

  • (P. lutherii)
  • (Ph. tricornutum)
  • Chlorophyll c
  • Light harvesting complex
  • Pigment protein complex
  • Secondary structure
  • Ultraviolet circular dichroism

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