Abstract
The light-harvesting complex and Photosystem I have been isolated from thylakoids of two chromophyte algae by digitonin solubilisation and sucrose-gradient centrifugation. The α-helic and β-structure content was determined by ultraviolet circular dichroism. The values obtained were approx. 40% α-helix and approx. 14% β-structure for the light-harvesting complex of both Pavlova lutherii and Phaeodactylum tricornutum. For Photosystem I the values were approx. 55% α-helix and 7% β-structure for both algae. It is concluded that for all photosynthetic antennae containing chlorophyll the dominant secondary structure is α-helix.
Original language | English |
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Pages (from-to) | 365-369 |
Number of pages | 5 |
Journal | BBA - Bioenergetics |
Volume | 894 |
Issue number | 3 |
DOIs | |
Publication status | Published - 17 Dec 1987 |
Externally published | Yes |
Keywords
- (P. lutherii)
- (Ph. tricornutum)
- Chlorophyll c
- Light harvesting complex
- Pigment protein complex
- Secondary structure
- Ultraviolet circular dichroism