The secondary structure content of pigment-protein complexes from the thylakoids of two Chromophyte algae

Roger G. Hiller; Anne Marie Bardin; Eliane Nabedryk

doi:10.1016/0005-2728(87)90114-9

The secondary structure content of pigment-protein complexes from the thylakoids of two Chromophyte algae

Roger G. Hiller^*, Anne Marie Bardin, Eliane Nabedryk

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The light-harvesting complex and Photosystem I have been isolated from thylakoids of two chromophyte algae by digitonin solubilisation and sucrose-gradient centrifugation. The α-helic and β-structure content was determined by ultraviolet circular dichroism. The values obtained were approx. 40% α-helix and approx. 14% β-structure for the light-harvesting complex of both Pavlova lutherii and Phaeodactylum tricornutum. For Photosystem I the values were approx. 55% α-helix and 7% β-structure for both algae. It is concluded that for all photosynthetic antennae containing chlorophyll the dominant secondary structure is α-helix.

Original language	English
Pages (from-to)	365-369
Number of pages	5
Journal	BBA - Bioenergetics
Volume	894
Issue number	3
DOIs	https://doi.org/10.1016/0005-2728(87)90114-9
Publication status	Published - 17 Dec 1987
Externally published	Yes

Keywords

(P. lutherii)
(Ph. tricornutum)
Chlorophyll c
Light harvesting complex
Pigment protein complex
Secondary structure
Ultraviolet circular dichroism

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10.1016/0005-2728(87)90114-9

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title = "The secondary structure content of pigment-protein complexes from the thylakoids of two Chromophyte algae",

abstract = "The light-harvesting complex and Photosystem I have been isolated from thylakoids of two chromophyte algae by digitonin solubilisation and sucrose-gradient centrifugation. The α-helic and β-structure content was determined by ultraviolet circular dichroism. The values obtained were approx. 40\% α-helix and approx. 14\% β-structure for the light-harvesting complex of both Pavlova lutherii and Phaeodactylum tricornutum. For Photosystem I the values were approx. 55\% α-helix and 7\% β-structure for both algae. It is concluded that for all photosynthetic antennae containing chlorophyll the dominant secondary structure is α-helix.",

keywords = "(P. lutherii), (Ph. tricornutum), Chlorophyll c, Light harvesting complex, Pigment protein complex, Secondary structure, Ultraviolet circular dichroism",

author = "Hiller, \{Roger G.\} and Bardin, \{Anne Marie\} and Eliane Nabedryk",

year = "1987",

month = dec,

day = "17",

doi = "10.1016/0005-2728(87)90114-9",

language = "English",

volume = "894",

pages = "365--369",

journal = "BBA - Bioenergetics",

issn = "0005-2728",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - The secondary structure content of pigment-protein complexes from the thylakoids of two Chromophyte algae

AU - Hiller, Roger G.

AU - Bardin, Anne Marie

AU - Nabedryk, Eliane

PY - 1987/12/17

Y1 - 1987/12/17

N2 - The light-harvesting complex and Photosystem I have been isolated from thylakoids of two chromophyte algae by digitonin solubilisation and sucrose-gradient centrifugation. The α-helic and β-structure content was determined by ultraviolet circular dichroism. The values obtained were approx. 40% α-helix and approx. 14% β-structure for the light-harvesting complex of both Pavlova lutherii and Phaeodactylum tricornutum. For Photosystem I the values were approx. 55% α-helix and 7% β-structure for both algae. It is concluded that for all photosynthetic antennae containing chlorophyll the dominant secondary structure is α-helix.

AB - The light-harvesting complex and Photosystem I have been isolated from thylakoids of two chromophyte algae by digitonin solubilisation and sucrose-gradient centrifugation. The α-helic and β-structure content was determined by ultraviolet circular dichroism. The values obtained were approx. 40% α-helix and approx. 14% β-structure for the light-harvesting complex of both Pavlova lutherii and Phaeodactylum tricornutum. For Photosystem I the values were approx. 55% α-helix and 7% β-structure for both algae. It is concluded that for all photosynthetic antennae containing chlorophyll the dominant secondary structure is α-helix.

KW - (P. lutherii)

KW - (Ph. tricornutum)

KW - Chlorophyll c

KW - Light harvesting complex

KW - Pigment protein complex

KW - Secondary structure

KW - Ultraviolet circular dichroism

UR - https://www.scopus.com/pages/publications/4243416943

U2 - 10.1016/0005-2728(87)90114-9

DO - 10.1016/0005-2728(87)90114-9

M3 - Article

AN - SCOPUS:4243416943

SN - 0005-2728

VL - 894

SP - 365

EP - 369

JO - BBA - Bioenergetics

JF - BBA - Bioenergetics

IS - 3

ER -

The secondary structure content of pigment-protein complexes from the thylakoids of two Chromophyte algae

Abstract

Keywords

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