TY - JOUR
T1 - The sequence and organization of Ddp2, a high-copy-number nuclear plasmid of Dictyostelium discoideum
AU - Slade, Martin B.
AU - Chang, Andy C. M.
AU - Williams, Keith L.
PY - 1990/11
Y1 - 1990/11
N2 - The complete nucleotide sequence of the plasmid Ddp2 found in the nucleus of the simple eukaryoteDictyostelium discoideum is reported. This 5852-bp plasmid contains a 2661-bp open reading frame (ORF), named the "Rep gene," and 501-bp imperfect inverted repeats. A 1762-bp section of Ddp2, which includes one of the 501-bp repeat sequences, could be deleted without abolishing extrachromosomal replication. Deletion of the second 501-bp repeat, or interruption of the Rep gene, removed the ability to replicate extrachromosomally. We suggest that Ddp2 encodes a protein, "REP," that positively regulates replication initiation, a regulatory mechanism different from that of the yeast 2μ plasmid which also possesses inverted repeat sequences. Ddp2 has a structure similar to that of plasmid pDG1, found in an unidentified isolate ofDictyostelium, with a similar sized ORF and inverted repeats. A common evolutionary origin is suggested by considerable sequence homology between the ORFs of pDG1 and Ddp2.
AB - The complete nucleotide sequence of the plasmid Ddp2 found in the nucleus of the simple eukaryoteDictyostelium discoideum is reported. This 5852-bp plasmid contains a 2661-bp open reading frame (ORF), named the "Rep gene," and 501-bp imperfect inverted repeats. A 1762-bp section of Ddp2, which includes one of the 501-bp repeat sequences, could be deleted without abolishing extrachromosomal replication. Deletion of the second 501-bp repeat, or interruption of the Rep gene, removed the ability to replicate extrachromosomally. We suggest that Ddp2 encodes a protein, "REP," that positively regulates replication initiation, a regulatory mechanism different from that of the yeast 2μ plasmid which also possesses inverted repeat sequences. Ddp2 has a structure similar to that of plasmid pDG1, found in an unidentified isolate ofDictyostelium, with a similar sized ORF and inverted repeats. A common evolutionary origin is suggested by considerable sequence homology between the ORFs of pDG1 and Ddp2.
UR - http://www.scopus.com/inward/record.url?scp=0025667996&partnerID=8YFLogxK
U2 - 10.1016/0147-619X(90)90003-U
DO - 10.1016/0147-619X(90)90003-U
M3 - Article
C2 - 2077544
AN - SCOPUS:0025667996
VL - 24
SP - 195
EP - 207
JO - Plasmid
JF - Plasmid
SN - 0147-619X
IS - 3
ER -