TY - JOUR
T1 - The stabilization of episomal integration by genetic inversion
T2 - a general hypothesis
AU - Adelberg, Edward A.
AU - Bergquist, Peter
PY - 1972/8
Y1 - 1972/8
N2 - In F′ cells of Escherichia coli K12, recombination in the region of genetic duplication between chromosome and F-genote produces a rapid alteration between the integrated and detached states of the episome. Nevertheless, F′ strains can give rise to Hfr cells in which the F-genote has undergone stable integration. We have found that one such Hfr has retained the genetic duplication as well as the recombination proficiency of the parental F′ strain; to account for the failure of recombination to detach the episome in this Hfr, we have postulated the inversion of one of the duplicated regions. A general model is presented, showing that an inversion must prevent episome detachment by any combination of genetic crossovers.
AB - In F′ cells of Escherichia coli K12, recombination in the region of genetic duplication between chromosome and F-genote produces a rapid alteration between the integrated and detached states of the episome. Nevertheless, F′ strains can give rise to Hfr cells in which the F-genote has undergone stable integration. We have found that one such Hfr has retained the genetic duplication as well as the recombination proficiency of the parental F′ strain; to account for the failure of recombination to detach the episome in this Hfr, we have postulated the inversion of one of the duplicated regions. A general model is presented, showing that an inversion must prevent episome detachment by any combination of genetic crossovers.
UR - http://www.scopus.com/inward/record.url?scp=0015385342&partnerID=8YFLogxK
U2 - 10.1073/pnas.69.8.2061
DO - 10.1073/pnas.69.8.2061
M3 - Article
C2 - 4559592
AN - SCOPUS:0015385342
SN - 0027-8424
VL - 69
SP - 2061
EP - 2065
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 8
ER -