In F′ cells of Escherichia coli K12, recombination in the region of genetic duplication between chromosome and F-genote produces a rapid alteration between the integrated and detached states of the episome. Nevertheless, F′ strains can give rise to Hfr cells in which the F-genote has undergone stable integration. We have found that one such Hfr has retained the genetic duplication as well as the recombination proficiency of the parental F′ strain; to account for the failure of recombination to detach the episome in this Hfr, we have postulated the inversion of one of the duplicated regions. A general model is presented, showing that an inversion must prevent episome detachment by any combination of genetic crossovers.
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|Publication status||Published - Aug 1972|